Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers

Parkson L. Chong, Mei Mei Wang, Fang Liu, Khanh Truong, Anthony A. Golsorkhi, Istvan P. Sugar, Rhoderick E Brown

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Our previous studies indicated that sterols (including cholesterol and dehydroergosterol) can be regularly distributed into hexagonal superlattices in the plane of liquid-crystalline phosphatidylcholine bilayers. It was suggested that regular and irregular regions coexist in the membrane. In the present study, we report supporting evidence for our sterol regular distribution model. We have examined the fractional concentration dependencies of dehydroergosterol (a naturally occurring cholesterol analog) fluorescence intensity and lifetime in various phosphatidylcholine and sphingomyelin bilayers. Fluorescence intensity and lifetime dips have been observed at specific sterol mole fractions. At those mole fractions, the acrylamide quenching rate constant of dehydroergosterol fluorescence reaches a local maximum. Those mole fractions match with the critical sterol mole fractions at which sterol molecules are expected to be regularly distributed into hexagonal superlattices. The results support the idea that the sterols in the regular region are embedded in the bilayer less deep than those in the irregular regions. Furthermore, finding evidence for sterol regular distribution in both phosphatidylcholine and sphingomyelin membranes raises the possibility that sterol regular distribution may occur within phospholipid/cholesterol enriched domains of real biological membranes.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsE.R. Menzel
Pages143-154
Number of pages12
Volume2705
StatePublished - Jan 1 1996
Externally publishedYes
EventFluorescence Detection IV - San Jose, CA, USA
Duration: Feb 1 1996Feb 2 1996

Other

OtherFluorescence Detection IV
CitySan Jose, CA, USA
Period2/1/962/2/96

Fingerprint

Lipid bilayers
Cholesterol
cholesterol
lipids
Fluorescence
Superlattices
fluorescence
membranes
Membranes
Biological membranes
superlattices
Phospholipids
life (durability)
Quenching
Rate constants
Crystalline materials
Molecules
quenching
Liquids
analogs

Cite this

Chong, P. L., Wang, M. M., Liu, F., Truong, K., Golsorkhi, A. A., Sugar, I. P., & Brown, R. E. (1996). Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers. In E. R. Menzel (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2705, pp. 143-154)

Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers. / Chong, Parkson L.; Wang, Mei Mei; Liu, Fang; Truong, Khanh; Golsorkhi, Anthony A.; Sugar, Istvan P.; Brown, Rhoderick E.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / E.R. Menzel. Vol. 2705 1996. p. 143-154.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chong, PL, Wang, MM, Liu, F, Truong, K, Golsorkhi, AA, Sugar, IP & Brown, RE 1996, Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers. in ER Menzel (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2705, pp. 143-154, Fluorescence Detection IV, San Jose, CA, USA, 2/1/96.
Chong PL, Wang MM, Liu F, Truong K, Golsorkhi AA, Sugar IP et al. Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers. In Menzel ER, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2705. 1996. p. 143-154
Chong, Parkson L. ; Wang, Mei Mei ; Liu, Fang ; Truong, Khanh ; Golsorkhi, Anthony A. ; Sugar, Istvan P. ; Brown, Rhoderick E. / Fluorescence evidence for cholesterol regular distribution in phosphatidylcholine and in sphingomyeline lipid bilayers. Proceedings of SPIE - The International Society for Optical Engineering. editor / E.R. Menzel. Vol. 2705 1996. pp. 143-154
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