Fluorescence energy transfer efficiency in labeled yeast cytochrome c: A rapid screen for ion biocompatibility in aqueous ionic liquids

Sheila N. Baker, Hua Zhao, Siddharth Pandey, William T. Heller, Frank V. Bright, Gary A. Baker

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A fluorescence energy transfer "de-quenching" assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.

Original languageEnglish (US)
Pages (from-to)3642-3644
Number of pages3
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number9
DOIs
StatePublished - Mar 7 2011
Externally publishedYes

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