In general, the plasmid copy number and plasmid segregation properties are strongly influenced if not completely determined by plasmid functions. While considerable information is available on different replication origins and segregation functions in E. coli plasmids, relatively little information is available on yeast plasmids and the sequences which regulate replication and segregation. In the research reported, a powerful new experimental method based upon flow cytometry is applied to investigate two different recombination yeast strains. One harbors a plasmid in which replication function is provided by a cloned sequence from the yeast 2- mu m plasmid. A cloned chromosomal autonomous replication sequence (ARS) provides autonomous plasmid replication function in the second strain.
|Original language||English (US)|
|Title of host publication||Unknown Host Publication Title|
|Number of pages||6|
|State||Published - Dec 1 1984|