FLOW CYTOMETRY CHARACTERIZATION OF PLASMID STABILITY IN SACCHAROMYCES CEREVISIAE.

F. Srienc, J. Campbell, J. Bailey

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In general, the plasmid copy number and plasmid segregation properties are strongly influenced if not completely determined by plasmid functions. While considerable information is available on different replication origins and segregation functions in E. coli plasmids, relatively little information is available on yeast plasmids and the sequences which regulate replication and segregation. In the research reported, a powerful new experimental method based upon flow cytometry is applied to investigate two different recombination yeast strains. One harbors a plasmid in which replication function is provided by a cloned sequence from the yeast 2- mu m plasmid. A cloned chromosomal autonomous replication sequence (ARS) provides autonomous plasmid replication function in the second strain.

Original languageEnglish (US)
Title of host publicationUnknown Host Publication Title
PublisherVerlag Chemie
Pages187-192
Number of pages6
ISBN (Print)3527262822
StatePublished - Dec 1 1984

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  • Cite this

    Srienc, F., Campbell, J., & Bailey, J. (1984). FLOW CYTOMETRY CHARACTERIZATION OF PLASMID STABILITY IN SACCHAROMYCES CEREVISIAE. In Unknown Host Publication Title (pp. 187-192). Verlag Chemie.