Abstract
Flow cytometry was applied to measure the fluorescence of IgG molecules on hybridoma cell surface stained with FITC-conjugated anti-mouse IgG F(ab′)2. Using light scattering to exclude the dead cell population from the analysis, the surface fluorescence intensity allows for the differentiation between producing and nonproducing cells. A linear correlation has been found between the intensity of mean surface fluorescence of the cell population and the specific antibody production rate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 571-576 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 12 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1990 |
Keywords
- Hybridoma
- cell culture
- flow cytometry
- monoclonal antibody