Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate

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37 Scopus citations

Abstract

Flow cytometry was applied to measure the fluorescence of IgG molecules on hybridoma cell surface stained with FITC-conjugated anti-mouse IgG F(ab′)2. Using light scattering to exclude the dead cell population from the analysis, the surface fluorescence intensity allows for the differentiation between producing and nonproducing cells. A linear correlation has been found between the intensity of mean surface fluorescence of the cell population and the specific antibody production rate.

Original languageEnglish (US)
Pages (from-to)571-576
Number of pages6
JournalEnzyme and Microbial Technology
Volume12
Issue number8
DOIs
StatePublished - Aug 1990

Keywords

  • Hybridoma
  • cell culture
  • flow cytometry
  • monoclonal antibody

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