Conditions for flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in infected mosquito cells are described. This approach will streamline investigation of Wolbachia's interactions with host cells and facilitate identification of culture conditions that select for Wolbachia-infected cells.
Bibliographical noteFunding Information:
This work was supported by NIH grant AI 081322 and by the University of Minnesota Agricultural Experiment Station, St. Paul, MN . I thank Drs. T.J. Kurtti and U.G. Munderloh for helpful advice.
- Flow cytometry
- Intracellular microorganism
- Mosquito cell line