FLIM microscopy with a streak camera

Monitoring metabolic processes in living cells and tissues

V. Krishnan Ramanujan, Javier A. Jo, Ravi Ranjan, Brian Herman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A critical understanding of subcellular phenomena—from the perspectives of various metabolic processes—stems from a strategic alliance between technological innovations and elegant model systems. Intravital fluorescence imaging modalities rely on exciting specific fluorophores in cells and tissues and interpreting the measured fluorescence emission signals in relation to the cellular processes that can modulate these signals (Richards-Kortum and Sevick-Muraca 1996). A recent upsurge in multiple modes, including spectral, lifetime, polarization etc., has shed valuable information on the various aspects of molecular–cellular and physiological complexity (Lyons 2005; Pawley 2006; Periasamy 2001).

Original languageEnglish (US)
Title of host publicationFlim Microscopy in Biology and Medicine
PublisherCRC Press
Pages189-209
Number of pages21
ISBN (Electronic)9781420078916
ISBN (Print)9781420078909
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Inventions
Streak cameras
streak cameras
Optical Imaging
Microscopy
Microscopic examination
Fluorescence
Cells
Tissue
microscopy
fluorescence
Fluorophores
Monitoring
Innovation
Polarization
Imaging techniques
life (durability)
polarization
cells
Intravital Microscopy

Cite this

Krishnan Ramanujan, V., Jo, J. A., Ranjan, R., & Herman, B. (2009). FLIM microscopy with a streak camera: Monitoring metabolic processes in living cells and tissues. In Flim Microscopy in Biology and Medicine (pp. 189-209). CRC Press.

FLIM microscopy with a streak camera : Monitoring metabolic processes in living cells and tissues. / Krishnan Ramanujan, V.; Jo, Javier A.; Ranjan, Ravi; Herman, Brian.

Flim Microscopy in Biology and Medicine. CRC Press, 2009. p. 189-209.

Research output: Chapter in Book/Report/Conference proceedingChapter

Krishnan Ramanujan, V, Jo, JA, Ranjan, R & Herman, B 2009, FLIM microscopy with a streak camera: Monitoring metabolic processes in living cells and tissues. in Flim Microscopy in Biology and Medicine. CRC Press, pp. 189-209.
Krishnan Ramanujan V, Jo JA, Ranjan R, Herman B. FLIM microscopy with a streak camera: Monitoring metabolic processes in living cells and tissues. In Flim Microscopy in Biology and Medicine. CRC Press. 2009. p. 189-209
Krishnan Ramanujan, V. ; Jo, Javier A. ; Ranjan, Ravi ; Herman, Brian. / FLIM microscopy with a streak camera : Monitoring metabolic processes in living cells and tissues. Flim Microscopy in Biology and Medicine. CRC Press, 2009. pp. 189-209
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