Flexible and disposable immunosensors based on layer-by-layer self-assembled carbon nanotubes and biomolecules

Miao Lu, Dongjin Lee, Wei Xue, Tianhong Cui

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.

Original languageEnglish (US)
Pages (from-to)280-285
Number of pages6
JournalSensors and Actuators, A: Physical
Volume150
Issue number2
DOIs
StatePublished - Mar 25 2009

Keywords

  • Immunosensor
  • Layer-by-layer (LbL) self-assembly
  • MEMS
  • Single-walled carbon nanotube (SWNT)

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