Five year maintenance of the inducible expression of anthranilate synthase in catharanthus roseus hairy roots

Christie A M Peebles, Guy W. Sander, Mai Li, Jacqueline V. Shanks, Ka Yiu San

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Transgenic hairy root cultures have the potential to be an industrial production platform for a variety of chemicals. This report demonstrates the long-term stability of a transgenic Catharanthus roseus hairy root line containing the inducible expression of a feedback-insensitive anthranilate synthase (AS). After 5 years in liquid culture, the presence of the inserted AS gene was confirmed by genomic PCR. The inducible expression of AS was confirmed by enzyme assay and by changes in terpenoid indole alkaloid concentrations. This report also demonstrates that it may take as long as 2 years for the metabolite profile to stabilize.

Original languageEnglish (US)
Pages (from-to)1521-1525
Number of pages5
JournalBiotechnology and Bioengineering
Volume102
Issue number5
DOIs
StatePublished - Apr 1 2009

Fingerprint

Anthranilate Synthase
Catharanthus
Production platforms
Alkaloids
Metabolites
Cell culture
Assays
Enzymes
Genes
Maintenance
Feedback
Liquids
Secologanin Tryptamine Alkaloids
Enzyme Assays
Polymerase Chain Reaction

Keywords

  • Cell factories
  • Inducible promoter
  • Plant metabolic engineering
  • Terpenoid indole alkaloid

Cite this

Five year maintenance of the inducible expression of anthranilate synthase in catharanthus roseus hairy roots. / Peebles, Christie A M; Sander, Guy W.; Li, Mai; Shanks, Jacqueline V.; San, Ka Yiu.

In: Biotechnology and Bioengineering, Vol. 102, No. 5, 01.04.2009, p. 1521-1525.

Research output: Contribution to journalArticle

Peebles, Christie A M ; Sander, Guy W. ; Li, Mai ; Shanks, Jacqueline V. ; San, Ka Yiu. / Five year maintenance of the inducible expression of anthranilate synthase in catharanthus roseus hairy roots. In: Biotechnology and Bioengineering. 2009 ; Vol. 102, No. 5. pp. 1521-1525.
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