Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein

Kai Wu, Vinit Kumar Chugh, Venkatramana D. Krishna, Yongqiang Andrew Wang, Timothy D. Gordon, Maxim C.J. Cheeran, Jian Ping Wang

Research output: Contribution to journalArticlepeer-review


In this work, we report a 5-min magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface-functionalized magnetic nanoparticles are incubated with target analytes at 37 °C with agitation for 3 min, and the MPS reading is then taken at the fifth minute. We prove the feasibility of 5 min ultrafast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmol). Our proposed 5-min bioassay strategy may be applied to reduce the assay time for other liquid-phase, volumetric biosensors such as NMR, quantum dots, fluorescent biosensors, etc.

Original languageEnglish (US)
Pages (from-to)17503-17507
Number of pages5
JournalACS Applied Nano Materials
Issue number12
StatePublished - Dec 23 2022

Bibliographical note

Funding Information:
This study was financially supported by the Institute of Engineering in Medicine, the Robert F. Hartmann Endowed Chair professorship, the University of Minnesota Medical School, and the University of Minnesota Physicians and Fairview Health Services through a COVID-19 Rapid Response Grant. This study was also financially supported by the U.S. Department of Agriculture, National Institute of Food and Agriculture, under Award 2020-67021-31956. Research reported in this publication was supported by the National Institute of Dental & Craniofacial Research of the National Institutes of Health under Award R42DE030832. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Portions of this work were conducted in the Minnesota Nano Center, which is supported by the National Science Foundation through the National Nano Coordinated Infrastructure Network under Award ECCS-1542202.

Publisher Copyright:
© 2022 American Chemical Society.


  • SARS-CoV-2
  • bioassay
  • magnetic nanoparticle
  • magnetic particle spectroscopy
  • spike protein
  • volumetric biosensor

PubMed: MeSH publication types

  • Journal Article


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