First trimester phthalate exposure and anogenital distance in newborns

S. H. Swan, S. Sathyanarayana, E. S. Barrett, S. Janssen, F. Liu, R. H.N. Nguyen, J. B. Redmon

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236 Scopus citations


Study Question Is first trimester phthalate exposure associated with anogenital distance (AGD), a biomarker of prenatal androgen exposure, in newborns? Summary Answer Concentrations of diethylhexyl phthalate (DEHP) metabolites in first trimester maternal urine samples are inversely associated with AGD in male, but not female, newborns. What is Known Already AGD is a sexually dimorphic measure reflecting prenatal androgen exposure. Prenatal phthalate exposure has been associated with shorter male AGD in multiple animal studies. Prior human studies, which have been limited by small sample size and imprecise timing of exposure and/or outcome, have reported conflicting results. Study Design, Size, Duration The Infant Development and the Environment Study (TIDES) is a prospective cohort study of pregnant women recruited in prenatal clinics in San Francisco, CA, Minneapolis, MN, Rochester, NY and Seattle, WA in 2010-2012. Participants delivered 787 infants; 753 with complete data are included in this analysis. Participants/Materials, Setting, Methods Any woman over 18 years old who was able to read and write English (or Spanish in CA), who was <13 weeks pregnant, whose pregnancy was not medically threatened and who planned to deliver in a study hospital was eligible to participate. Analyses include all infants whose mothers provided a first trimester urine sample and who were examined at or shortly after birth. Specific gravity (SpG) adjusted concentrations of phthalate metabolites in first trimester urine samples were examined in relation to genital measurements. In boys (N = 366), we obtained two measures of anogenital distance (AGD) (anoscrotal distance, or AGD AS and anopenile distance, AGD AP) as well as penile width (PW). In girls (N = 373), we measured anofourchette distance (AGD AF) and anoclitoral distance (AGD AC). We used multivariable regression models that adjusted for the infant's age at exam, gestational age, weight-for-length Z-score, time of day of urine collection, maternal age and study center. Main Results and The Role of Chance Three metabolites of DEHP were significantly and inversely associated with both measures of boys' AGD. Associations (β 2, 95% confidence interval (CI)) between AGD AS and (log 10) SpG-adjusted phthalate concentrations were: â '1.12 (â '2.16, â '0.07) for mono-2-ethylhexyl phthalate (MEHP), â'1.43, (â '2.49, â '0.38) for mono-2-ethyl-5-oxohexyl phthalate (MEOHP), and â '1.28 (â '2.29, â '0.27) for mono-2-ethyl-5-hydroxyhexyl (MEHHP). Associations were of similar magnitude for AGD AP. Associations were weaker and not statistically significant for PW. No other phthalate metabolites were associated with any genital measurement in boys. No phthalate metabolites were associated with either AGD measure in girls. Limitations, Reasons For Caution Exposure assessment was based on a single first trimester urine sample, which may have introduced exposure misclassification. In addition, significant between-center differences suggest that this measurement is difficult to standardize. Wider Implications of The Findings Our findings are consistent with multiple rodent studies and most human studies which were far smaller. The data we report here suggest that even at current low levels, environmental exposure to DEHP can adversely affect male genital development resulting in reproductive tract changes that may impact reproductive health later in life. These findings have important implications for public policy since most pregnant women are exposed to this ubiquitous chemical. Study Funding/Competing Interest(S) Funding for TIDES was provided by the following grants from the National Institute of Environmental Health Sciences: R01ES016863-04 and R01 ES016863-02S4. The authors report no conflict of interest.

Original languageEnglish (US)
Pages (from-to)963-972
Number of pages10
JournalHuman Reproduction
Issue number4
StatePublished - Sep 30 2015

Bibliographical note

Funding Information:
Funding for TIDES was provided by the following grants from the National Institute of Environmental Health Sciences: R01ES016863-04 and R01 ES016863-02S4.

Publisher Copyright:
© 2015 The Author. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.


  • anogenital distance
  • environmental chemicals
  • phthalate syndrome
  • phthalates
  • prenatal development


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