First report of porcine respirovirus 1 in South America

B. Agüero, J. Mena, F. Berrios, R. Tapia, C. Salinas, J. Dutta, H. van Bakel, S. K. Mor, B. Brito, R. A. Medina, V. Neira

Research output: Contribution to journalArticle

Abstract

Porcine respirovirus 1 (PRV1) is an emerging virus in pigs that has been previously described in the USA and China. There are no reports of its presence in the rest of the world. The objective of this study was to determine the occurrence of PRV1 in Chile and to determine its phylogeny. Thus, we collected samples (oral fluids, nasal swabs, and lungs) from a swine influenza A virus (IAV) surveillance program, most of which belonged to pigs with respiratory disease. The samples were analyzed by RT-PCR, and the viral sequencing was obtained using RNA whole-genome sequencing approach. Maximum likelihood phylogeny was constructed with the available references. Thirty-one of 164 samples (18.9 %) were RT-PCR positive for PRV1: 62.5 % oral fluids, 19.0 % nasal swabs, and 8.6 % lungs. All 6 farms in this study had at least one positive sample, with 6–40 % of positive results per farm, which suggests that PRV1 is disseminated in Chilean swine farms. Twenty-one of 31 (677%) PRV1-positive samples were also positive for IAV, so the role of PRV1 as secondary pathogen in respiratory disease needs to be further evaluated. Near to complete genome of two PRV1s were obtained from two farms. The phylogenies, in general, showed low bootstrap support, except the concatenated genome and the L gene trees which showed clustering of the Chilean PRV1 with Asian sequences, suggesting a close genetic relationship. This is the first report of PRV1 in the Southern Hemisphere. Further studies are necessary to determine the genetic diversity of this virus in Chile.

Original languageEnglish (US)
Article number108726
JournalVeterinary Microbiology
Volume246
DOIs
StatePublished - Jul 2020
Externally publishedYes

Keywords

  • Chile
  • Porcine parainfluenza virus 1
  • Porcine respirovirus 1
  • South America
  • Swine

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'First report of porcine respirovirus 1 in South America'. Together they form a unique fingerprint.

  • Cite this

    Agüero, B., Mena, J., Berrios, F., Tapia, R., Salinas, C., Dutta, J., van Bakel, H., Mor, S. K., Brito, B., Medina, R. A., & Neira, V. (2020). First report of porcine respirovirus 1 in South America. Veterinary Microbiology, 246, [108726]. https://doi.org/10.1016/j.vetmic.2020.108726