First report of bacterial leaf streak caused by xanthomonas translucens pv. undulosa on cultivated wild rice (zizania palustris) in minnesota

R. D. Curland, K. R. Hallada, K. E. Ledman, R. Dill-Macky

Research output: Contribution to journalComment/debatepeer-review

Abstract

Known by the indigenous peoples of the Great Lakes region of North America as Manoomin, wild rice ( Zizania palustris) is a native aquatic grass that is honored and central to Anishinaabe culture. Cultivated wild rice, the domesticated form of this cereal bred primarily for resistance to shattering, is grown commercially in paddies. In this study we examined four isolates (CIX303, CIX306, Xt-8, and Xt-22) of Xanthomonas translucens, the causative agent of bacterial leaf streak (BLS) on cereals and weedy grasses, in molecular and host range studies to confirm the pathovar identity of strains associated with cultivated wild rice. Two of the strains examined (CIX303 and CIX306), were isolated from cultivated wild rice in 2016 as part of a survey of the pathogen in Minnesota (Ledman 2019). Xt-8 and Xt-22 are historical strains of X. translucens isolated from symptomatic wild rice leaves collected in Minnesota in the late 1970s that were reported at the time to be X. campestris pv. cerealis (Bowden and Percich 1982). A host range assay was repeated twice in the greenhouse, where two leaves of six seedlings each of hard red spring wheat (cv. RB07), spring barley (cv. Quest), spring rye (cv. Prolific), oat (cv. Ogle), quackgrass, smooth brome grass and cultivated wild rice (cv. Itasca Cycle-12) were inoculated via leaf infiltration (Curland et al. 2020). X. translucens pv. cerealis LMG 679 PT, X. translucens pv. secalis LMG 883 PT, X. translucens pv. translucens LMG 876 T, and X. translucens pv. undulosa LMG 892 PT were included as reference strains. Host response profiles were determined for each strain by recording character states five days post inoculation. Water-soaking and necrosis were considered pathogenic reactions, whereas chlorosis was not. Three pathotype strains, LMG 679 PT, LMG 876 T, and LMG 892 PT, caused water-soaking in cultivated wild rice, whereas LMG 883 PT caused chlorosis. All four strains from cultivated wild rice produced water-soaking on wheat, barley, quackgrass, and cultivated wild rice, chlorosis or water-soaking on rye, chlorosis on oat, and a reddish water-soaking on smooth brome. The character states generated by these four isolates were identical only to the host response profile for LMG 892 PT. LMG 679 PT differed, causing chlorosis on wheat, no symptoms on quackgrass, and water-soaking on smooth brome. A 2645 bp concatenation of housekeeping genes ( rpoD, dnaK, fyuA, gyrB) was used to perform a Bayesian analysis (GenBank accessions MW528365-MW528384) (Curland et al. 2018, Curland et al. 2020, Young et al. 2008). Subsequent phylogenies grouped all four strains from cultivated wild rice with LMG 892 PT and LMG 883 PT. A pairwise comparison revealed 100% identity between Xt-22 and LMG 892 PT. The percentage identity of CIX303, CIX308, and Xt-8 to LMG 892 PT was 99.96, 99.96, and 99.92, respectively. In contrast, when compared to LMG 679 PT, the four strains from cultivated wild rice had a percent identity between 97.43 and 97.50. Based on host range studies combined with MLSA, we identified recent and historical isolates from Z. palustris as X. translucens pv. undulosa. Pathovar identity of strains causing BLS on cultivated wild rice in Minnesota is crucial when screening breeding materials for disease resistance. Furthermore, given that X. translucens pv. undulosa has been prevalent on wheat in Minnesota (Curland et al. 2018), expanding knowledge of its host range to include cultivated wild rice may inform disease management practices for both crops. References: Bowden, R., and Percich, J. 1982. Phytopath. 73:640-645. Curland, R., et al. 2018. Phytopath. 108:443-453. Curland, R., et al. 2020. Phytopath. 110:257-266. Ledman, K. 2019. M.S. Thesis, Univ. of Minnesota, St. P. Paul, USA. Young, J., et al. 2008. Syst. Appl. Microbiol. 31:366-377.

Original languageEnglish (US)
JournalPlant disease
Volume105
Issue number9
DOIs
StatePublished - Sep 2021

Keywords

  • Cereals and grains
  • Field crops
  • Pathogen detection
  • Prokaryotes

PubMed: MeSH publication types

  • Journal Article

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