First record of resting cysts of the benthic dinoflagellate Prorocentrum leve in a natural reservoir in Gujan-Mestras, Gironde, France

Kenneth Neil Mertens, Haifeng Gu, Vera Pospelova, Nicolas Chomérat, Elisabeth Nézan, Pieter Roger Gurdebeke, Kara Bogus, Henk Vrielinck, Myriam Rumebe, Claire Meteigner

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7 Scopus citations

Abstract

The resting cysts of the benthic dinoflagellate Prorocentrum leve from a natural reservoir in Gujan-Mestras (Gironde, France) were described in this study. The incubated urn-shaped cysts gave rise to cells of P. leve. Morphological observations through light microscopy and scanning electron microscopy, particularly of the periflagellar platelets, combined with large subunit ribosomal DNA sequences obtained through single-cell analysis confirm their affinity to the species P. leve. The cysts were characterized by a specific shape and the presence of an anterior plug. This is the first conclusive evidence for fossilizable resting stages within the Prorocentrales, one of the major orders within the Dinophyceae. Palynological treatments show that the cysts and endospores withstand hydrochloric and hydrofluoric acids. Micro-Fourier transform infrared analysis on single specimens suggests that the composition of the endospore is cellulosic and the cyst wall a more robust, noncellulosic β-glucan. The spectra overall are similar to other published spectra of resting cysts from autotrophic, planktonic dinoflagellates.

Original languageEnglish (US)
Pages (from-to)1193-1205
Number of pages13
JournalJournal of Phycology
Volume53
Issue number6
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
Rob Fensome, Graham Williams, and Jim Riding are thanked for useful discussions regarding the cyst morphology. N. Gayet is acknowledged for critical-point drying of the samples for SEM. This research was partly supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) through a Discovery grant to VP. The Hercules Foundation (Flanders) is gratefully acknowledged for financial support (FT-IMAGER project - AUGE/13/16).

Keywords

  • Prorocentrales
  • cellulosic
  • micro-FTIR
  • palynology
  • resting stage

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