Findings on T cell specificity revealed by synthetic combinatorial libraries

Eva Borràs, Roland Martin, Valeria Judkowski, Jacqueline Shukaliak, Yingdong Zhao, Verena Rubio-Godoy, Danila Valmori, Darcy Wilson, Richard Simon, Richard Houghten, Clemencia Pinilla

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Combinatorial libraries and in particular positional scanning synthetic combinatorial libraries (PS-SCL) allow the study of T cell specificity. This is a systematic and unbiased approach that does not require any previous knowledge about the clones to be studied, neither their specificity nor they major histocompatibility complex (MHC) restriction. Two different types of T cell clone ligands can be identified: (1) peptides that do not necessarily correspond to proteins described in the databases, and (2) peptides that are fragments of natural proteins. In this paper, relevant examples of the application of PS-SCL and the deconvolution strategies followed to identify T cell epitopes for clones of known and unknown specificity will be reviewed. Also, important issues like the immunogenicity of such T cell ligands will be discussed.

Original languageEnglish (US)
Pages (from-to)79-97
Number of pages19
JournalJournal of Immunological Methods
Volume267
Issue number1
DOIs
StatePublished - Sep 1 2002
Externally publishedYes

Keywords

  • Biometrical analysis
  • Positional scanning synthetic combinatorial libraries
  • Protein database
  • T cell specificity

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