TY - JOUR
T1 - Findings on T cell specificity revealed by synthetic combinatorial libraries
AU - Borràs, Eva
AU - Martin, Roland
AU - Judkowski, Valeria
AU - Shukaliak, Jacqueline
AU - Zhao, Yingdong
AU - Rubio-Godoy, Verena
AU - Valmori, Danila
AU - Wilson, Darcy
AU - Simon, Richard
AU - Houghten, Richard
AU - Pinilla, Clemencia
PY - 2002/9/1
Y1 - 2002/9/1
N2 - Combinatorial libraries and in particular positional scanning synthetic combinatorial libraries (PS-SCL) allow the study of T cell specificity. This is a systematic and unbiased approach that does not require any previous knowledge about the clones to be studied, neither their specificity nor they major histocompatibility complex (MHC) restriction. Two different types of T cell clone ligands can be identified: (1) peptides that do not necessarily correspond to proteins described in the databases, and (2) peptides that are fragments of natural proteins. In this paper, relevant examples of the application of PS-SCL and the deconvolution strategies followed to identify T cell epitopes for clones of known and unknown specificity will be reviewed. Also, important issues like the immunogenicity of such T cell ligands will be discussed.
AB - Combinatorial libraries and in particular positional scanning synthetic combinatorial libraries (PS-SCL) allow the study of T cell specificity. This is a systematic and unbiased approach that does not require any previous knowledge about the clones to be studied, neither their specificity nor they major histocompatibility complex (MHC) restriction. Two different types of T cell clone ligands can be identified: (1) peptides that do not necessarily correspond to proteins described in the databases, and (2) peptides that are fragments of natural proteins. In this paper, relevant examples of the application of PS-SCL and the deconvolution strategies followed to identify T cell epitopes for clones of known and unknown specificity will be reviewed. Also, important issues like the immunogenicity of such T cell ligands will be discussed.
KW - Biometrical analysis
KW - Positional scanning synthetic combinatorial libraries
KW - Protein database
KW - T cell specificity
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U2 - 10.1016/S0022-1759(02)00142-4
DO - 10.1016/S0022-1759(02)00142-4
M3 - Article
C2 - 12135802
AN - SCOPUS:18444410234
SN - 0022-1759
VL - 267
SP - 79
EP - 97
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -