Fiber alignment imaging during mechanical testing of soft tissues

Theodore T. Tower; Michael R. Neidert; Robert T. Tranquillo

doi:10.1114/1.1527047

Fiber alignment imaging during mechanical testing of soft tissues

Theodore T. Tower, Michael R. Neidert, Robert T. Tranquillo

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

A novel method to dynamically image fiber alignment during mechanical testing, allowing direct correlation of mechanical properties to microstructural changes was developed. To demonstrate the capabilities of this system, both native tissue and tissue equivalents were tested. By testing both fresh and fixed tissues, it was possible to ascertain the effect of fiber alignment on loading. A combination of alignment imaging and preconditioning protocol enabled to determine the changes in the microstructure during preconditioning.

Original language	English (US)
Pages (from-to)	1221-1233
Number of pages	13
Journal	Annals of Biomedical Engineering
Volume	30
Issue number	10
DOIs	https://doi.org/10.1114/1.1527047
State	Published - 2002

Bibliographical note

Funding Information:
The NSF MRSEC-Artificial Tissues Program (DMR-9809364) primarily supported this work. Technical assistance of Dan Asleson and Luca Gunther and heart valve leaflets provided by Dr. Paul Iaizzo are also gratefully acknowledged.

Keywords

Glutaraldehyde fixation
Imaging
Polarized light microscopy
Tissue fiber alignment
Tissue mechanical testing
Tissue-equivalent

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abstract = "A novel method to dynamically image fiber alignment during mechanical testing, allowing direct correlation of mechanical properties to microstructural changes was developed. To demonstrate the capabilities of this system, both native tissue and tissue equivalents were tested. By testing both fresh and fixed tissues, it was possible to ascertain the effect of fiber alignment on loading. A combination of alignment imaging and preconditioning protocol enabled to determine the changes in the microstructure during preconditioning.",

keywords = "Glutaraldehyde fixation, Imaging, Polarized light microscopy, Tissue fiber alignment, Tissue mechanical testing, Tissue-equivalent",

author = "Tower, {Theodore T.} and Neidert, {Michael R.} and Tranquillo, {Robert T.}",

note = "Funding Information: The NSF MRSEC-Artificial Tissues Program (DMR-9809364) primarily supported this work. Technical assistance of Dan Asleson and Luca Gunther and heart valve leaflets provided by Dr. Paul Iaizzo are also gratefully acknowledged.",

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AU - Tower, Theodore T.

AU - Neidert, Michael R.

AU - Tranquillo, Robert T.

N1 - Funding Information: The NSF MRSEC-Artificial Tissues Program (DMR-9809364) primarily supported this work. Technical assistance of Dan Asleson and Luca Gunther and heart valve leaflets provided by Dr. Paul Iaizzo are also gratefully acknowledged.

PY - 2002

Y1 - 2002

N2 - A novel method to dynamically image fiber alignment during mechanical testing, allowing direct correlation of mechanical properties to microstructural changes was developed. To demonstrate the capabilities of this system, both native tissue and tissue equivalents were tested. By testing both fresh and fixed tissues, it was possible to ascertain the effect of fiber alignment on loading. A combination of alignment imaging and preconditioning protocol enabled to determine the changes in the microstructure during preconditioning.

AB - A novel method to dynamically image fiber alignment during mechanical testing, allowing direct correlation of mechanical properties to microstructural changes was developed. To demonstrate the capabilities of this system, both native tissue and tissue equivalents were tested. By testing both fresh and fixed tissues, it was possible to ascertain the effect of fiber alignment on loading. A combination of alignment imaging and preconditioning protocol enabled to determine the changes in the microstructure during preconditioning.

KW - Glutaraldehyde fixation

KW - Imaging

KW - Polarized light microscopy

KW - Tissue fiber alignment

KW - Tissue mechanical testing

KW - Tissue-equivalent

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ER -

Fiber alignment imaging during mechanical testing of soft tissues

Abstract

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Keywords

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