Fermentation profiles of wheat dextrin, Inulin and partially hydrolyzed guar gum using an in Vitro digestion pretreatment and in Vitro batch fermentation system model

Jackie Noack, Derek Timm, Ashok Hospattankar, Joanne L Slavin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study investigated the fermentation and microbiota profiles of three fibers, wheat dextrin (WD), partially hydrolyzed guar gum (PHGG), and inulin, since little is known about the effects of WD and PHGG on gut microbiota. A treatment of salivary amylase, pepsin, and pancreatin was used to better physiologic digestion. Fibers (0.5 g) were fermented in triplicate including a control group without fiber for 0, 4, 8, 12, and 24 h. Analysis of pH, gas volume, hydrogen and methane gases, and short chain fatty acid (SCFA) concentrations were completed at each time point. Quantitative polymerase chain reaction (qPCR) was used to measure Bifidobacteria and Lactobacillus CFUs at 24 h. WD produced the least gas during fermentation at 8, 12, and 24 h (P < 0.0001), while inulin produced the most by 8 h (P < 0.0001). Each fiber reached its lowest pH value at different time points with inulin at 8 h (mean ± SE) (5.94 ± 0.03), PHGG at 12 h (5.98 ± 0.01), and WD at 24 h (6.17 ± 0.03). All fibers had higher total SCFA concentrations compared to the negative control (P < 0.05) at 24 h. At 24 h, inulin produced significantly (P = 0.0016) more butyrate than WD with PHGG being similar to both. An exploratory microbial analysis (log10 CFU/μL) showed WD had CFU for Bifidobacteria (6.12) and Lactobacillus (7.15) compared with the control (4.92 and 6.35, respectively). Rate of gas production is influenced by fiber source and may affect tolerance in vivo. Exploratory microbiota data at high levels of Bifidobacteria for WD, but require more robust investigation to corroborate these findings.

Original languageEnglish (US)
Pages (from-to)1500-1510
Number of pages11
JournalNutrients
Volume5
Issue number5
DOIs
StatePublished - May 3 2013

Fingerprint

guar gum
batch fermentation
dextrins
in vitro digestion
Inulin
inulin
Triticum
Fermentation
Digestion
pretreatment
fermentation
dietary fiber
wheat
Bifidobacterium
Gases
Volatile Fatty Acids
Microbiota
gases
Lactobacillus
short chain fatty acids

Keywords

  • In vitro fermentation
  • Microbiota
  • Prebiotic
  • Short-chain fatty acids
  • Tolerance

Cite this

Fermentation profiles of wheat dextrin, Inulin and partially hydrolyzed guar gum using an in Vitro digestion pretreatment and in Vitro batch fermentation system model. / Noack, Jackie; Timm, Derek; Hospattankar, Ashok; Slavin, Joanne L.

In: Nutrients, Vol. 5, No. 5, 03.05.2013, p. 1500-1510.

Research output: Contribution to journalArticle

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abstract = "This study investigated the fermentation and microbiota profiles of three fibers, wheat dextrin (WD), partially hydrolyzed guar gum (PHGG), and inulin, since little is known about the effects of WD and PHGG on gut microbiota. A treatment of salivary amylase, pepsin, and pancreatin was used to better physiologic digestion. Fibers (0.5 g) were fermented in triplicate including a control group without fiber for 0, 4, 8, 12, and 24 h. Analysis of pH, gas volume, hydrogen and methane gases, and short chain fatty acid (SCFA) concentrations were completed at each time point. Quantitative polymerase chain reaction (qPCR) was used to measure Bifidobacteria and Lactobacillus CFUs at 24 h. WD produced the least gas during fermentation at 8, 12, and 24 h (P < 0.0001), while inulin produced the most by 8 h (P < 0.0001). Each fiber reached its lowest pH value at different time points with inulin at 8 h (mean ± SE) (5.94 ± 0.03), PHGG at 12 h (5.98 ± 0.01), and WD at 24 h (6.17 ± 0.03). All fibers had higher total SCFA concentrations compared to the negative control (P < 0.05) at 24 h. At 24 h, inulin produced significantly (P = 0.0016) more butyrate than WD with PHGG being similar to both. An exploratory microbial analysis (log10 CFU/μL) showed WD had CFU for Bifidobacteria (6.12) and Lactobacillus (7.15) compared with the control (4.92 and 6.35, respectively). Rate of gas production is influenced by fiber source and may affect tolerance in vivo. Exploratory microbiota data at high levels of Bifidobacteria for WD, but require more robust investigation to corroborate these findings.",
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