Feline immunodeficiency virus-mediated gene therapy of middle ear mucosa cells

Hamid R. Djalilian, Yasuhiro Tsuboi, Masashi Ozeki, Muhammet Tekin, Ali R. Djalilian, Wesley Obritch, Jizhen Lin

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Hypothesis: To investigate the feasibility of gene therapy of the middle ear mucosa using a novel vector. Background: Given present medications are unable to affect chronic otitis media, cholesteatoma, or tympanic membrane perforation, newer methods of treatment like gene therapy for these diseases must be explored. These genes can then be used to alter cytokines in the middle ear, slow or stop cholesteatoma growth, or improve tympanic membrane perforation healing. Feline immunodeficiency virus (FIV), a new lentiviral vector has been found to have greater than 90% efficiency in transfecting epithelial cells. Therefore, in vivo gene therapy of middle ear mucosa cells was attempted. Methods: Twenty microliter of 5×105 vectors per ml FIV carrying the gene for green fluorescence protein (GFP) was introduced into the middle ears of Sprague-Dawley rats via a bulla approach. Results: Expression of the GFP gene was observed in the middle ear mucosa cells at 1 week post-inoculation indicating transfection. Conclusion: Gene therapy of the middle ear is feasible with a FIV-based vector.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalAuris Nasus Larynx
Volume29
Issue number2
DOIs
StatePublished - Mar 26 2002

Keywords

  • Feline
  • GFP gene
  • Mucosa cells

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    Djalilian, H. R., Tsuboi, Y., Ozeki, M., Tekin, M., Djalilian, A. R., Obritch, W., & Lin, J. (2002). Feline immunodeficiency virus-mediated gene therapy of middle ear mucosa cells. Auris Nasus Larynx, 29(2), 183-186. https://doi.org/10.1016/S0385-8146(01)00131-6