Feedback regulation of NEUROG2 activity by MTGR1 is required for progression of neurogenesis

Joshua D. Aaker, Andrea L. Patineau, Hyun jin Yang, David T. Ewart, Wuming Gong, Tongbin Li, Yasushi Nakagawa, Steven C. McLoon, Naoko Koyano-Nakagawa

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The sequential steps of neurogenesis are characterized by highly choreographed changes in transcription factor activity. In contrast to the well-studied mechanisms of transcription factor activation during neurogenesis, much less is understood regarding how such activity is terminated. We previously showed that MTGR1, a member of the MTG family of transcriptional repressors, is strongly induced by a proneural basic helix-loop-helix transcription factor, NEUROG2 in developing nervous system. In this study, we describe a novel feedback regulation of NEUROG2 activity by MTGR1. We show that MTGR1 physically interacts with NEUROG2 and represses transcriptional activity of NEUROG2. MTGR1 also prevents DNA binding of the NEUROG2/E47 complex. In addition, we provide evidence that proper termination of NEUROG2 activity by MTGR1 is necessary for normal progression of neurogenesis in the developing spinal cord. These results highlight the importance of feedback regulation of proneural gene activity in neurodevelopment.

Original languageEnglish (US)
Pages (from-to)267-277
Number of pages11
JournalMolecular and Cellular Neuroscience
Volume42
Issue number4
DOIs
StatePublished - Nov 2009

Keywords

  • Development
  • Embryo
  • MTG/ETO proteins
  • MTGR1
  • NEUROG2
  • Nervy
  • Neurogenesis
  • Spinal cord
  • Transcription
  • Transcriptional repressor
  • bHLH

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