Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer

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5 Scopus citations

Abstract

Chronic inflammation of the colon (colitis) is a known risk factor for inflammatory-driven colorectal cancers (id-CRCs), and intestinal microbiota has been implicated in the etiology of id-CRCs. Manipulation of the microbiome is a clinically viable therapeutic approach to limiting id-CRCs. To understand the microbiome changes that occur over time in id-CRCs, we used a mouse model of id-CRCs with the treatment of azoxymethane (AOM) and dextran sodium sulfate (DSS) and measured the microbiome over time. We included cohorts where the microbiome was restored using cage bedding swapping and where the microbiome was depleted using antibiotics to compare to untreated animals. We identified consistent increases in Akkermansia in mice receiving horizontal microbiome transfer (HMT) via cage bedding swapping, while the control cohort had consistent longitudinal increases in Anaeroplasma and Alistipes. Additionally, fecal lipocalin-2 (Lcn-2), a marker of intestinal inflammation, was elevated in unrestored animals compared to restored and antibiotic-treated counterparts following HMT. These observations suggest a potential role for Akkermansia, Anaeroplasma, and Alistipes in regulating colonic inflammation in id-CRCs.

Original languageEnglish (US)
Article number2260
JournalCancers
Volume15
Issue number8
DOIs
StatePublished - Apr 2023

Bibliographical note

Funding Information:
We thank the Masonic Cancer Center’s core facilities, the University of Minnesota Genomics Center, and the Clinical and Translational Sciences Institute for microbiome sequencing and supporting histological services. This study is supported by research grants from the Masonic Cancer Center ChainBreaker Fund, the Mezin Koats colorectal cancer research fund, Minnesota Colorectal Cancer Funds, and research funds from the Department of Surgery and CTSI, University of Minnesota. The Minnesota Colorectal Cancer Research Foundation supports T.J.G. through a graduate fellowship.

Publisher Copyright:
© 2023 by the authors.

Keywords

  • colitis
  • colorectal cancer
  • horizontal microbiota transfer
  • longitudinal sampling
  • microbiome

PubMed: MeSH publication types

  • Journal Article

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