Feasibility of microbially induced carbonate precipitation through a Chlorella-Sporosaricina co-culture system

Pinpin Xu; Hua Fan; Lijian Leng; Liangliang Fan; Shuhua Liu; Paul Chen; Wenguang Zhou

doi:10.1016/j.algal.2020.101831

Feasibility of microbially induced carbonate precipitation through a Chlorella-Sporosaricina co-culture system

Pinpin Xu, Hua Fan, Lijian Leng, Liangliang Fan, Shuhua Liu, Paul Chen, Wenguang Zhou

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Microbially induced carbonate precipitation, an eco-friendly and energy-efficient technology, has attracted many researchers' attention because of its potential use for soil solidification, crack repair and concrete self-repair, etc. In this study, the feasibility of using enhanced co-culture of Chlorella sp. and Sporosaricina pasteurii under mixotrophic conditions to induce carbonate precipitation was investigated. The effects of different growing conditions on microbial growth and their relationship with microbially induced carbonate precipitation were evaluated. The results showed that under optimal growth conditions of algae-bacteria inoculation volume ratio of 3:2 (v/v), initial pH value of 9.0 and glucose concentration of 1 g.L⁻¹, the algae-bacteria co-culture system was able to effectively induce carbonate precipitation and the Chlorella sp. biomass in the co-culture system was significantly improved by 37.74%. The Ca²⁺ rate was reduced by 60.4% in the co-culture system, which was higher than that in the mono-culture system. The calcification rate constant in the co-culture was 0.3514. The crystalline mineral particles were calcite crystals as determined by X-ray diffraction. It was observed through a scanning electron microscope that mineral particles piled up together and some microbial cells were deposited on the crystal surface. These results suggest that microbially induced carbonate precipitation through Chlorella-Sporosaricina co-culture is feasible, but more studies are necessary to understand mechanisms and optimize the process.

Original language	English (US)
Article number	101831
Journal	Algal Research
Volume	47
DOIs	https://doi.org/10.1016/j.algal.2020.101831
State	Published - May 2020

Bibliographical note

Funding Information:
This study was supported by the National Natural Science Foundation of China (31960734, 51668044), the Key Research Development Program of the Jiangxi Province of China (20171BBG70036 and 20181BBH80004), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China (20171BCB23015). PX and HF participated equally in designing, performing the experiments, analyzing the data and writing the initial draft; LJL, LLF, and SHL assisted in conducting the experiment and critical revision of the manuscript. PC and WGZ critically reviewed the manuscript. All authors read and approved the final version of the manuscript. All authors read and approved the final version of the manuscript.

Funding Information:
This study was supported by the National Natural Science Foundation of China ( 31960734 , 51668044 ), the Key Research Development Program of the Jiangxi Province of China ( 20171BBG70036 and 20181BBH80004 ), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China ( 20171BCB23015 ).

Publisher Copyright:
© 2020

Keywords

Calcification rate
Chlorella-Sporosaricina co-culture
Growth conditions optimization
Microbially induced carbonate precipitation
Shape and morphology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Feasibility of microbially induced carbonate precipitation through a Chlorella-Sporosaricina co-culture system",

abstract = "Microbially induced carbonate precipitation, an eco-friendly and energy-efficient technology, has attracted many researchers' attention because of its potential use for soil solidification, crack repair and concrete self-repair, etc. In this study, the feasibility of using enhanced co-culture of Chlorella sp. and Sporosaricina pasteurii under mixotrophic conditions to induce carbonate precipitation was investigated. The effects of different growing conditions on microbial growth and their relationship with microbially induced carbonate precipitation were evaluated. The results showed that under optimal growth conditions of algae-bacteria inoculation volume ratio of 3:2 (v/v), initial pH value of 9.0 and glucose concentration of 1 g.L−1, the algae-bacteria co-culture system was able to effectively induce carbonate precipitation and the Chlorella sp. biomass in the co-culture system was significantly improved by 37.74%. The Ca2+ rate was reduced by 60.4% in the co-culture system, which was higher than that in the mono-culture system. The calcification rate constant in the co-culture was 0.3514. The crystalline mineral particles were calcite crystals as determined by X-ray diffraction. It was observed through a scanning electron microscope that mineral particles piled up together and some microbial cells were deposited on the crystal surface. These results suggest that microbially induced carbonate precipitation through Chlorella-Sporosaricina co-culture is feasible, but more studies are necessary to understand mechanisms and optimize the process.",

keywords = "Calcification rate, Chlorella-Sporosaricina co-culture, Growth conditions optimization, Microbially induced carbonate precipitation, Shape and morphology",

author = "Pinpin Xu and Hua Fan and Lijian Leng and Liangliang Fan and Shuhua Liu and Paul Chen and Wenguang Zhou",

note = "Funding Information: This study was supported by the National Natural Science Foundation of China (31960734, 51668044), the Key Research Development Program of the Jiangxi Province of China (20171BBG70036 and 20181BBH80004), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China (20171BCB23015). PX and HF participated equally in designing, performing the experiments, analyzing the data and writing the initial draft; LJL, LLF, and SHL assisted in conducting the experiment and critical revision of the manuscript. PC and WGZ critically reviewed the manuscript. All authors read and approved the final version of the manuscript. All authors read and approved the final version of the manuscript. Funding Information: This study was supported by the National Natural Science Foundation of China ( 31960734 , 51668044 ), the Key Research Development Program of the Jiangxi Province of China ( 20171BBG70036 and 20181BBH80004 ), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China ( 20171BCB23015 ). Publisher Copyright: {\textcopyright} 2020",

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AU - Xu, Pinpin

AU - Fan, Hua

AU - Leng, Lijian

AU - Fan, Liangliang

AU - Liu, Shuhua

AU - Chen, Paul

AU - Zhou, Wenguang

N1 - Funding Information: This study was supported by the National Natural Science Foundation of China (31960734, 51668044), the Key Research Development Program of the Jiangxi Province of China (20171BBG70036 and 20181BBH80004), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China (20171BCB23015). PX and HF participated equally in designing, performing the experiments, analyzing the data and writing the initial draft; LJL, LLF, and SHL assisted in conducting the experiment and critical revision of the manuscript. PC and WGZ critically reviewed the manuscript. All authors read and approved the final version of the manuscript. All authors read and approved the final version of the manuscript. Funding Information: This study was supported by the National Natural Science Foundation of China ( 31960734 , 51668044 ), the Key Research Development Program of the Jiangxi Province of China ( 20171BBG70036 and 20181BBH80004 ), and the Talent Program for Distinguished Young Scholars of Jiangxi Province of China ( 20171BCB23015 ). Publisher Copyright: © 2020

PY - 2020/5

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N2 - Microbially induced carbonate precipitation, an eco-friendly and energy-efficient technology, has attracted many researchers' attention because of its potential use for soil solidification, crack repair and concrete self-repair, etc. In this study, the feasibility of using enhanced co-culture of Chlorella sp. and Sporosaricina pasteurii under mixotrophic conditions to induce carbonate precipitation was investigated. The effects of different growing conditions on microbial growth and their relationship with microbially induced carbonate precipitation were evaluated. The results showed that under optimal growth conditions of algae-bacteria inoculation volume ratio of 3:2 (v/v), initial pH value of 9.0 and glucose concentration of 1 g.L−1, the algae-bacteria co-culture system was able to effectively induce carbonate precipitation and the Chlorella sp. biomass in the co-culture system was significantly improved by 37.74%. The Ca2+ rate was reduced by 60.4% in the co-culture system, which was higher than that in the mono-culture system. The calcification rate constant in the co-culture was 0.3514. The crystalline mineral particles were calcite crystals as determined by X-ray diffraction. It was observed through a scanning electron microscope that mineral particles piled up together and some microbial cells were deposited on the crystal surface. These results suggest that microbially induced carbonate precipitation through Chlorella-Sporosaricina co-culture is feasible, but more studies are necessary to understand mechanisms and optimize the process.

AB - Microbially induced carbonate precipitation, an eco-friendly and energy-efficient technology, has attracted many researchers' attention because of its potential use for soil solidification, crack repair and concrete self-repair, etc. In this study, the feasibility of using enhanced co-culture of Chlorella sp. and Sporosaricina pasteurii under mixotrophic conditions to induce carbonate precipitation was investigated. The effects of different growing conditions on microbial growth and their relationship with microbially induced carbonate precipitation were evaluated. The results showed that under optimal growth conditions of algae-bacteria inoculation volume ratio of 3:2 (v/v), initial pH value of 9.0 and glucose concentration of 1 g.L−1, the algae-bacteria co-culture system was able to effectively induce carbonate precipitation and the Chlorella sp. biomass in the co-culture system was significantly improved by 37.74%. The Ca2+ rate was reduced by 60.4% in the co-culture system, which was higher than that in the mono-culture system. The calcification rate constant in the co-culture was 0.3514. The crystalline mineral particles were calcite crystals as determined by X-ray diffraction. It was observed through a scanning electron microscope that mineral particles piled up together and some microbial cells were deposited on the crystal surface. These results suggest that microbially induced carbonate precipitation through Chlorella-Sporosaricina co-culture is feasible, but more studies are necessary to understand mechanisms and optimize the process.

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KW - Chlorella-Sporosaricina co-culture

KW - Growth conditions optimization

KW - Microbially induced carbonate precipitation

KW - Shape and morphology

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Feasibility of microbially induced carbonate precipitation through a Chlorella-Sporosaricina co-culture system

Abstract

Bibliographical note

Keywords

UN SDGs

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