Feasibility of hydrogen production from ripened fruits by a combined two-stage (dark/dark) fermentation system

Jae Hoon Hwang, Jeong A. Choi, Reda A.I. Abou-Shanab, Booki Min, Hocheol Song, Yongje Kim, Eung Seok Lee, Byong Hun Jeon

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Anaerobic fermentation for hydrogen (H2) production was studied in a two-stage fermentation system fed with different ripened fruit feedstocks (apple, pear, and grape). Among the feedstocks, ripened apple was the most efficient substrate for cumulative H2 production (4463.7mL-H2 L-1-culture) with a maximum H2 yield (2.2mol H2 mol-1 glucose) in the first stage at a hydraulic retention time (HRT) of 18h. The additional cumulative biohydrogen (3337.4mL-H2 L-1-culture) was produced in the second stage with the reused residual substrate from the first stage. The major byproducts in this study were butyrate, acetate, and ethanol, and butyrate was dominant among them in all test runs. During the two-stage system, the energy efficiency (H2 conversion) obtained from mixed ripened fruits (RF) increased from 4.6% (in the first stage) to 15.5% (in the second stage), which indicated the energy efficiency can be improved by combined hydrogen production process. The RF could be used as substrates for biohydrogen fermentation in a two-stage (dark/dark) fermentation system.

Original languageEnglish (US)
Pages (from-to)1051-1058
Number of pages8
JournalBioresource Technology
Issue number2
StatePublished - Jan 2011

Bibliographical note

Funding Information:
This work was supported by the Students’ Association of the Graduate School of Yonsei University and funded by the Graduate School of Yonsei University , 21st Frontier research project (Sustainable Water Resources Research Center 3-4-3), Global Research Laboratory project (Korea Institute of Geosciences and Mineral Resources NP2008-019), Korea Institute of Energy Research and Brain Korea-21 (BK-21) program of the Ministry of Education.


  • Biohydrogen
  • Energy efficiency
  • Hydraulic retention time
  • Ripened fruits
  • Two-stage fermentation


Dive into the research topics of 'Feasibility of hydrogen production from ripened fruits by a combined two-stage (dark/dark) fermentation system'. Together they form a unique fingerprint.

Cite this