Abstract
In this work, we study the influence of the mobile phase and column temperature on the enantioresolution of basic compounds on microparticulate porous zirconia coated with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC). The chiral analytes are amino compounds, including a number of β-blockers. Analytes are eluted with hexane-alcohol mobile phases. We investigated the effect of alcohol (type and concentration), basic eluent additives, and column temperature on the parameters that control resolution (column efficiency, retention and selectivity). Conditions for achieving an adequate separation in the least time have been determined for numerous racemic mixtures. For most solutes, baseline resolution of the enantiomeric pair was achieved in less than 1 min; 12 of 13 pairs were separated in less than 2 min. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-33 |
| Number of pages | 17 |
| Journal | Journal of Chromatography A |
| Volume | 904 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 22 2000 |
Bibliographical note
Funding Information:We acknowledge help from Ray Weigand at Alltech Associates for providing the packed columns, ZirChrom Separations Inc. for the gift of the uncoated zirconia, and the National Institutes of Health for financial support. C. B. C. acknowledges her fellowship from CONICET (Argentina).
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
Keywords
- Basic compounds
- Cellulose tris(3,5-dimethylphenylcarbamate)
- Chiral stationary phases, LC
- Enantiomer separation
- Mobile-phase composition
- β-Blockers