Family-selective detection of antibiotics using antibody-functionalized carbon nanotube sensors

Byeongju Kim, Donghyun Lim, Hye Jun Jin, Hyang Yeon Lee, Seon Namgung, Yeonjin Ko, Seung Bum Park, Seunghun Hong

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The development of a rapid and sensitive detection method for well-known, yet abused, antibiotics has been an important issue for food safety and environmental protection. This paper presents a simple and sensitive method for the specific or family-selective detection of antibiotics using carbon nanotube (CNT)-based sensors. Herein, CNT-based sensor transducers were functionalized with the single-chain variable-fragment (scFv) of antibodies that can selectively bind to a specific antibiotic or the certain family of antibiotics. Our CNT-based sensors functionalized with A2 scFv or F9 scFv exhibited the specific detection of enrofloxacin or the family-selective detection of fluoroquinolone-based antibiotics, respectively, in a real-time manner. This simple but efficient strategy can be utilized for various applications in the fields of food safety and environmental protection.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume166-167
DOIs
StatePublished - May 20 2012

Bibliographical note

Funding Information:
This work was supported by the WCU program (R31-2010-000-10032-0). S. H. acknowledges equipment supports from the NRF grant ( 20110000390 ) and Converging Research Center Program funded by the Ministry of Education, Science and Technology ( 2011K000683 ). S.B.P. acknowledges the support from National Research Foundation of Korea ( NRF-2009-0078236 ) and Chemical Genomics grant ( M10526020002-08N2602-00210 ).

Keywords

  • Antibiotic
  • Carbon nanotube
  • Enrofloxacin
  • Family-selective detection
  • Norfloxacin
  • Sensor

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