Abstract
PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.
Original language | English (US) |
---|---|
Pages (from-to) | 133-138 |
Number of pages | 6 |
Journal | Biologicals |
Volume | 37 |
Issue number | 3 |
DOIs | |
State | Published - Jun 2009 |
Externally published | Yes |
Bibliographical note
Funding Information:The research was funded in part by a grant from the Indiana 21st Century Fund.
Keywords
- Chlamydomonas
- Chloroplast transformation
- Recombinant protein expression