Factors effecting expression of vaccines in microalgae

Raymond Surzycki, Katie Greenham, Kaoru Kitayama, Flora Dibal, Richard Wagner, Jean David Rochaix, Tarek Ajam, Stefan Surzycki

Research output: Contribution to journalArticlepeer-review

127 Scopus citations


PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
Issue number3
StatePublished - Jun 2009
Externally publishedYes

Bibliographical note

Funding Information:
The research was funded in part by a grant from the Indiana 21st Century Fund.


  • Chlamydomonas
  • Chloroplast transformation
  • Recombinant protein expression


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