Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy

Ian L. Gunsolus, Dehong Hu, Cosmin Mihai, Samuel E. Lohse, Chang Soo Lee, Marco D. Torelli, Robert J. Hamers, Catherine J. Murhpy, Galya Orr, Christy L. Haynes

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A method to fluorescently stain the surfaces of both Gram-negative and Gram-positive bacterial cells compatible with super-resolution fluorescence microscopy is presented. This method utilizes a commercially-available fluorescent probe to label primary amines at the surface of the cell. We demonstrate efficient staining of two bacterial strains, the Gram-negative Shewanella oneidensis MR-1 and the Gram-positive Bacillus subtilis 168. Using structured illumination microscopy and stochastic optical reconstruction microscopy, which require high quantum yield or specialized dyes, we show that this staining method may be used to resolve the bacterial cell surface with sub-diffraction-limited resolution. We further use this method to identify localization patterns of nanomaterials, specifically cadmium selenide quantum dots, following interaction with bacterial cells.

Original languageEnglish (US)
Pages (from-to)3174-3178
Number of pages5
JournalAnalyst
Volume139
Issue number12
DOIs
StatePublished - Jun 19 2014

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