FABP3 and FABP10 in Atlantic salmon (Salmo salar L.)- General effects of dietary fatty acid composition and life cycle variations

Ann Elise O. Jordal, Ivar Hordvik, Maurice Pelsers, David A. Bernlohr, Bente E. Torstensen

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The increased use of dietary plant oil supplementation combined with high dietary lipid loads challenges the lipid transport systems of cultivated fish species. Fatty acid binding proteins (FABPs) have been thoroughly studied as intracellular fatty acid transporters in vertebrates, but no data have been reported in Atlantic salmon. In the present study, comparative characterizations were performed, and dietary influence of plant oil supplementation on FABP3 and FABP10 expression was studied for several tissues in two separate dietary trials. In trial I, groups (6 fish each) were fed diets for 42 weeks (body mass 142 ± 1 to 1463 ± 83 g) (mean ± S.D.), containing graded levels of rapeseed oil substituting for fish oil using a linear regression design. In trial II, groups (3 fish each) were fed 100% fish oil or 100% plant oil for 22 months (0.160 ± 0.052 to 2523 ± 590 g) (mean ± S.D.) and sampled at regular intervals. Liver and muscle tissues appeared to express several FABPs possibly linked to different metabolic functions. FABPs mRNA expression did not change with dietary inclusion of 75% rapeseed oil, whereas FABP3 protein expression seemed to be affected by dietary rapeseed oil inclusion. Significant changes in red muscle FABP3 mRNA expression correlate to significant changes in total β-oxidation capacity during the energy consuming process of smoltification.

Original languageEnglish (US)
Pages (from-to)147-158
Number of pages12
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume145
Issue number2
DOIs
StatePublished - Oct 2006

Bibliographical note

Funding Information:
This work was part of “RAFOA, Researching Alternatives to Fish Oils in Aquaculture”, Q5RS-2000-30058 funded by EU, The Fifth Framework Programme and NIH DK 053189 (Prof. David A. Bernlohr). The authors are obliged to Eva Mykkeltvedt and Betty Irgens for excellent technical assistance and help during samplings. We are greatly in debt to the staff of Gildeskaal and Lerang research station for their skilled work with husbandry. Dr. Pål Olsvik is thanked for fruitful discussions. Dr. Ann Hertzel is thanked for her help in the work of cloning cDNA encoding FABP3. Dr. Susan E. Douglas is thanked for letting us use the assumed FABP10 sequences for our work. The help of Dr. Dominic Nanton in reviewing the final document is greatly appreciated.

Keywords

  • Atlantic salmon
  • FABP
  • FABP1
  • FABP3
  • FABP4
  • FAPB10
  • Fatty acid oxidation
  • Fatty acids
  • Quantitative PCR

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