TY - JOUR
T1 - FAAP20
T2 - A novel ubiquitin-binding FA nuclear core-complex protein required for functional integrity of the FA-BRCA DNA repair pathway
AU - Ali, Abdullah Mahmood
AU - Pradhan, Arun
AU - Singh, Thiyam Ramsingh
AU - Du, Changhu
AU - Li, Jie
AU - Wahengbam, Kebola
AU - Grassman, Elke
AU - Auerbach, Arleen D.
AU - Pang, Qishen
AU - Meetei, Amom Ruhikanta
PY - 2012/4/5
Y1 - 2012/4/5
N2 - Fanconi anemia (FA) nuclear core complex is a multiprotein complex required for the functional integrity of the FA-BRCA pathway regulating DNA repair. This pathway is inactivated in FA, a devastating genetic disease, which leads to hematologic defects and cancer in patients. Here we report the isolation and characterization of a novel 20-kDa FANCA-associated protein (FAAP20). We show that FAAP20 is an integral component of the FA nuclear core complex. We identify a region on FANCA that physically interacts with FAAP20, and show that FANCA regulates stability of this protein. FAAP20 contains a conserved ubiquitin-binding zinc-finger domain (UBZ), and binds K-63-linked ubiquitin chains in vitro. The FAAP20-UBZ domain is not required for interaction with FANCA, but is required for DNA-damage-induced chromatin loading of FANCA and the functional integrity of the FA pathway. These findings reveal critical roles for FAAP20 in the FABRCA pathway of DNA damage repair and genome maintenance.
AB - Fanconi anemia (FA) nuclear core complex is a multiprotein complex required for the functional integrity of the FA-BRCA pathway regulating DNA repair. This pathway is inactivated in FA, a devastating genetic disease, which leads to hematologic defects and cancer in patients. Here we report the isolation and characterization of a novel 20-kDa FANCA-associated protein (FAAP20). We show that FAAP20 is an integral component of the FA nuclear core complex. We identify a region on FANCA that physically interacts with FAAP20, and show that FANCA regulates stability of this protein. FAAP20 contains a conserved ubiquitin-binding zinc-finger domain (UBZ), and binds K-63-linked ubiquitin chains in vitro. The FAAP20-UBZ domain is not required for interaction with FANCA, but is required for DNA-damage-induced chromatin loading of FANCA and the functional integrity of the FA pathway. These findings reveal critical roles for FAAP20 in the FABRCA pathway of DNA damage repair and genome maintenance.
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U2 - 10.1182/blood-2011-10-385963
DO - 10.1182/blood-2011-10-385963
M3 - Article
C2 - 22343915
AN - SCOPUS:84859564894
SN - 0006-4971
VL - 119
SP - 3285
EP - 3294
JO - Blood
JF - Blood
IS - 14
ER -