Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle, Conor D. Morrison, Nichole N. Barger

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalSoil Biology and Biochemistry
Volume43
Issue number4
DOIs
StatePublished - Apr 2011
Externally publishedYes

Bibliographical note

Funding Information:
The authors would like to thank Dr. William Adams for providing use of laboratory equipment. We would also like to thank Matthew Ross, Alisha Mercer, Drew Habig, and Nikolas Mirhashemi for their help with sample processing and analysis. This work was funded by a grant made to N. Barger through the USDA Cooperative State Research Extension and Education Services (CSREES) -Managed Ecosystems program.

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Keywords

  • Acetone
  • Biological soil crust
  • Chlorophyll a
  • DMSO
  • Dimethyl sulfoxide
  • Ethanol
  • Methanol

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