Abstract
We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.
Original language | English (US) |
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Pages (from-to) | 853-856 |
Number of pages | 4 |
Journal | Soil Biology and Biochemistry |
Volume | 43 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1 2011 |
Keywords
- Acetone
- Biological soil crust
- Chlorophyll a
- DMSO
- Dimethyl sulfoxide
- Ethanol
- Methanol