Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle, Conor D. Morrison, Nichole N. Barger

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalSoil Biology and Biochemistry
Volume43
Issue number4
DOIs
StatePublished - Apr 1 2011

Keywords

  • Acetone
  • Biological soil crust
  • Chlorophyll a
  • DMSO
  • Dimethyl sulfoxide
  • Ethanol
  • Methanol

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