We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.
Bibliographical noteFunding Information:
The authors would like to thank Dr. William Adams for providing use of laboratory equipment. We would also like to thank Matthew Ross, Alisha Mercer, Drew Habig, and Nikolas Mirhashemi for their help with sample processing and analysis. This work was funded by a grant made to N. Barger through the USDA Cooperative State Research Extension and Education Services (CSREES) -Managed Ecosystems program.
Copyright 2011 Elsevier B.V., All rights reserved.
- Biological soil crust
- Chlorophyll a
- Dimethyl sulfoxide