Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle; Conor D. Morrison; Nichole N. Barger

doi:10.1016/j.soilbio.2010.11.025

Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle, Conor D. Morrison, Nichole N. Barger

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

Original language	English (US)
Pages (from-to)	853-856
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	43
Issue number	4
DOIs	https://doi.org/10.1016/j.soilbio.2010.11.025
State	Published - Apr 2011
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Dr. William Adams for providing use of laboratory equipment. We would also like to thank Matthew Ross, Alisha Mercer, Drew Habig, and Nikolas Mirhashemi for their help with sample processing and analysis. This work was funded by a grant made to N. Barger through the USDA Cooperative State Research Extension and Education Services (CSREES) -Managed Ecosystems program.

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Keywords

Acetone
Biological soil crust
Chlorophyll a
DMSO
Dimethyl sulfoxide
Ethanol
Methanol

Publisher link

10.1016/j.soilbio.2010.11.025

Find It

Find It at U of M Libraries

Cite this

@article{ec997747114443c9b52bfc2531e4c977,

title = "Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination",

abstract = "We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.",

keywords = "Acetone, Biological soil crust, Chlorophyll a, DMSO, Dimethyl sulfoxide, Ethanol, Methanol",

author = "Castle, {Sarah C.} and Morrison, {Conor D.} and Barger, {Nichole N.}",

note = "Funding Information: The authors would like to thank Dr. William Adams for providing use of laboratory equipment. We would also like to thank Matthew Ross, Alisha Mercer, Drew Habig, and Nikolas Mirhashemi for their help with sample processing and analysis. This work was funded by a grant made to N. Barger through the USDA Cooperative State Research Extension and Education Services (CSREES) -Managed Ecosystems program. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

month = apr,

doi = "10.1016/j.soilbio.2010.11.025",

language = "English (US)",

volume = "43",

pages = "853--856",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Extraction of chlorophyll a from biological soil crusts

T2 - A comparison of solvents for spectrophotometric determination

AU - Castle, Sarah C.

AU - Morrison, Conor D.

AU - Barger, Nichole N.

N1 - Funding Information: The authors would like to thank Dr. William Adams for providing use of laboratory equipment. We would also like to thank Matthew Ross, Alisha Mercer, Drew Habig, and Nikolas Mirhashemi for their help with sample processing and analysis. This work was funded by a grant made to N. Barger through the USDA Cooperative State Research Extension and Education Services (CSREES) -Managed Ecosystems program. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/4

Y1 - 2011/4

N2 - We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

AB - We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

KW - Acetone

KW - Biological soil crust

KW - Chlorophyll a

KW - DMSO

KW - Dimethyl sulfoxide

KW - Ethanol

KW - Methanol

UR - http://www.scopus.com/inward/record.url?scp=79951683421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951683421&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2010.11.025

DO - 10.1016/j.soilbio.2010.11.025

M3 - Article

AN - SCOPUS:79951683421

SN - 0038-0717

VL - 43

SP - 853

EP - 856

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

IS - 4

ER -

Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this