Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle; Conor D. Morrison; Nichole N. Barger

doi:10.1016/j.soilbio.2010.11.025

Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

Sarah C. Castle, Conor D. Morrison, Nichole N. Barger

Plant Pathology

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

Original language	English (US)
Pages (from-to)	853-856
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	43
Issue number	4
DOIs	https://doi.org/10.1016/j.soilbio.2010.11.025
State	Published - Apr 1 2011

Keywords

Acetone
Biological soil crust
Chlorophyll a
DMSO
Dimethyl sulfoxide
Ethanol
Methanol

Access

10.1016/j.soilbio.2010.11.025

Fingerprint Dive into the research topics of 'Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination'. Together they form a unique fingerprint.

Cite this

@article{ec997747114443c9b52bfc2531e4c977,

title = "Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination",

abstract = "We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.",

keywords = "Acetone, Biological soil crust, Chlorophyll a, DMSO, Dimethyl sulfoxide, Ethanol, Methanol",

author = "Castle, {Sarah C.} and Morrison, {Conor D.} and Barger, {Nichole N.}",

year = "2011",

month = apr,

day = "1",

doi = "10.1016/j.soilbio.2010.11.025",

language = "English (US)",

volume = "43",

pages = "853--856",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Extraction of chlorophyll a from biological soil crusts

T2 - A comparison of solvents for spectrophotometric determination

AU - Castle, Sarah C.

AU - Morrison, Conor D.

AU - Barger, Nichole N.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

AB - We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chl. a from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chl. a recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chl. a using a two-extraction efficiency calculation.

KW - Acetone

KW - Biological soil crust

KW - Chlorophyll a

KW - DMSO

KW - Dimethyl sulfoxide

KW - Ethanol

KW - Methanol

UR - http://www.scopus.com/inward/record.url?scp=79951683421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951683421&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2010.11.025

DO - 10.1016/j.soilbio.2010.11.025

M3 - Article

AN - SCOPUS:79951683421

VL - 43

SP - 853

EP - 856

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 4

ER -