Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy

Erdogan Pekcan Erkan, Nurten Saydam, Clark C Chen, Okay Saydam

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gene therapy involves the introduction of genes (termed transgenes) into cells to compensate for a deficiency or to make a beneficial protein. Gene therapy can used as a form of cancer treatment. A particularly attractive paradigm in this regard involves the selective introduction of transgenes into cancer cells that converts inactive prodrugs into active chemotherapeutic agents, thereby triggering the death of cancer cells. Since prodrugs are inactive, they tend not to cause significant side-effects and are well-tolerated by patients relative to conventional chemotherapy. Several viral and nonviral vectors have been used as delivery tools for suicide gene therapy. Extracellular vesicles (EVs) are now recognized as a promising class of nonviral delivery vectors. Here, we describe a method in which a suicide fusion gene construct is loaded into EVs derived from a non-tumorigenic cell line. Delivery of these modified EVs to glioblastoma cell lines and spheroids decreases glioblastoma cell viability, induces apoptotic cell death, and inhibits tumor growth in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages87-96
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1895
ISSN (Print)1064-3745

Fingerprint

Suicide
Genetic Therapy
Messenger RNA
Prodrugs
Glioblastoma
Transgenes
Neoplasms
Proteins
Cell Death
Cell Line
Gene Fusion
Therapeutics
Cell Survival
Drug Therapy
Extracellular Vesicles
Growth
Genes

Keywords

  • Cancer therapy
  • Extracellular vesicles
  • Glioblastoma
  • Suicide mRNA
  • Suicide protein

PubMed: MeSH publication types

  • Journal Article

Cite this

Erkan, E. P., Saydam, N., Chen, C. C., & Saydam, O. (2019). Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy. In Methods in Molecular Biology (pp. 87-96). (Methods in Molecular Biology; Vol. 1895). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8922-5_7

Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy. / Erkan, Erdogan Pekcan; Saydam, Nurten; Chen, Clark C; Saydam, Okay.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 87-96 (Methods in Molecular Biology; Vol. 1895).

Research output: Chapter in Book/Report/Conference proceedingChapter

Erkan, EP, Saydam, N, Chen, CC & Saydam, O 2019, Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1895, Humana Press Inc., pp. 87-96. https://doi.org/10.1007/978-1-4939-8922-5_7
Erkan EP, Saydam N, Chen CC, Saydam O. Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 87-96. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8922-5_7
Erkan, Erdogan Pekcan ; Saydam, Nurten ; Chen, Clark C ; Saydam, Okay. / Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 87-96 (Methods in Molecular Biology).
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