Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development

Heven Sze, Senthilkumar Padmanaban, Françoise Cellier, David Honys, Ning Hui Cheng, Kevin W. Bock, Genevieve Conéjéro, Xiyan Li, David Twell, John M. Ward, Kendal D. Hirschi

Research output: Contribution to journalArticle

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Abstract

A combined bioinformatic and experimental approach is being used to uncover the functions of a novel family of cation/H+ exchanger (CHX) genes in plants using Arabidopsis as a model. The predicted protein (85-95 kD) of 28 AtCHX genes after revision consists of an amino-terminal domain with 10 to 12 transmembrane spans (approximately 440 residues) and a hydrophilic domain of approximately 360 residues at the carboxyl end, which is proposed to have regulatory roles. The hydrophobic, but not the hydrophilic, domain of plant CHX is remarkably similar to monovalent cation/proton antiporter-2 (CPA2) proteins, especially yeast (Saccharomyces cerevisiae) KHA1 and Synechocystis NhaS4. Reports of characterized fungal and prokaryotic CPA2 indicate that they have various transport modes, including K+/H+ (KHA1), Na +/H+-K+ (GerN) antiport, and ligand-gated ion channel (KefC). The expression pattern of AtCHX genes was determined by reverse transcription PCR, promoter-driven β-glucuronidase expression in transgenic plants, and Affymetrix ATH1 genome arrays. Results show that 18 genes are specifically or preferentially expressed in the male gametophyte, and six genes are highly expressed in sporophytic tissues. Microarray data revealed that several AtCHX genes were developmentally regulated during microgametogenesis. An exciting idea is that CHX proteins allow osmotic adjustment and K+ homeostasis as mature pollen desiccates and then rehydrates at germination. The multiplicity of CHX-like genes is conserved in higher plants but is not found in animals. Only 17 genes, OsCHX01 to OsCHX17, were identified in rice (Oryza sativa) subsp. japonica, suggesting diversification of CHX in Arabidopsis. These results reveal a novel CHX gene family in flowering plants with potential functions in pollen development, germination, and tube growth.

Original languageEnglish (US)
Pages (from-to)2532-2547
Number of pages16
JournalPlant physiology
Volume136
Issue number1
DOIs
StatePublished - Sep 2004

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Pollen
homeostasis
Homeostasis
pollen
Cations
cations
Antiporters
Genes
genes
antiporters
Germination
Arabidopsis
Protons
Ligand-Gated Ion Channels
Synechocystis
Monovalent Cations
Plant Genes
germination
Fungal Proteins
Genetically Modified Plants

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Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development. / Sze, Heven; Padmanaban, Senthilkumar; Cellier, Françoise; Honys, David; Cheng, Ning Hui; Bock, Kevin W.; Conéjéro, Genevieve; Li, Xiyan; Twell, David; Ward, John M.; Hirschi, Kendal D.

In: Plant physiology, Vol. 136, No. 1, 09.2004, p. 2532-2547.

Research output: Contribution to journalArticle

Sze, H, Padmanaban, S, Cellier, F, Honys, D, Cheng, NH, Bock, KW, Conéjéro, G, Li, X, Twell, D, Ward, JM & Hirschi, KD 2004, 'Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development', Plant physiology, vol. 136, no. 1, pp. 2532-2547. https://doi.org/10.1104/pp.104.046003
Sze, Heven ; Padmanaban, Senthilkumar ; Cellier, Françoise ; Honys, David ; Cheng, Ning Hui ; Bock, Kevin W. ; Conéjéro, Genevieve ; Li, Xiyan ; Twell, David ; Ward, John M. ; Hirschi, Kendal D. / Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development. In: Plant physiology. 2004 ; Vol. 136, No. 1. pp. 2532-2547.
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AU - Cheng, Ning Hui

AU - Bock, Kevin W.

AU - Conéjéro, Genevieve

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