Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells

Yan Zhang, Huaiyun Han, William F. Elmquist, Donald W. Miller

Research output: Contribution to journalArticlepeer-review

201 Scopus citations

Abstract

Multidrug resistance-associated protein (MRP) actively transports a broad range of anionic compounds out of the cell. To date, six different homologues of MRP (i.e. MRP1-MRP6) have been identified. The current study examines the expression of the various MRP homologues in both primary cultured bovine brain microvessel endothelial cells (BBMEC) and the capillary-enriched fraction from bovine brain homogenates. RT-PCR analysis demonstrated the presence of MRP1, MRP4, MRP5 and MRP6 in both BBMEC and the capillary-enriched fractions of brain homogenates. While low levels of MRP3 were detected in the BBMEC, it was not observed in the capillary-enriched fraction. In addition, RT-PCR and Western blot studies indicated an absence of MRP2 expression in both blood-brain barrier preparations. The presence of several different MRP homologues in the brain microvessel endothelial cells may be important in controlling the permeability of the blood-brain barrier to organic anions.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalBrain Research
Volume876
Issue number1-2
DOIs
StatePublished - Sep 8 2000
Externally publishedYes

Bibliographical note

Funding Information:
The current study was supported by NIH Grants R15-NSOD35364 (D.W.M.) and R29-CA75466 (W.F.E.). The authors wish to thank Dr Ann Hubbard for the WIF-B cells used in the present study.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Blood-brain barrier
  • Brain microvessel
  • Multidrug resistance-associated protein
  • Reverse transcriptase-polymerase chain reaction
  • Western blot

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