Expression of uncoupling protein-2 remains increased within hibernating myocardium despite successful coronary artery bypass grafting at 4 wk post-revascularization

Christopher T. Holley, Cayla M. Duffy, Tammy A. Butterick, Eric K. Long, Megan E. Lindsey, Jesús A. Cabrera, Herbert B. Ward, Edward O. McFalls, Rosemary F. Kelly

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

CONCLUSIONS: Despite patent grafts, RHM demonstrates a submaximal response to dobutamine infusion and increased mitochondrial UCP-2 expression. These data support the notion that recovery of the mitochondria in RHM is delayed early post-CABG and may contribute to impaired oxygen consumption and contractile reserve during catecholamine challenges.

BACKGROUND: We have previously shown that mitochondrial uncoupling protein-2 (UCP-2) is increased in a swine model of hibernating myocardium (HM). Although UCP-2 reduces oxidant stress, it can promote inefficiency of the electron transport chain. In this study, we tested whether UCP-2 remains increased in revascularized HM (RHM) after coronary artery bypass grafting (CABG).

METHODS: Seven swine underwent thoracotomy with placement of a constrictor on the left anterior descending artery (LAD). Twelve weeks later, a left internal mammary artery graft was placed on the distal LAD. Four weeks post-CABG, computed tomography angiography documented patent grafts and function. At the terminal study, blood flow to the LAD and remote territories were assessed during high dose dobutamine and mitochondria isolated from both regions for analysis. Comparisons were made to a group of swine with HM who underwent constrictor placement without bypass grafting (n = 4).

RESULTS: During dobutamine infusion, RHM demonstrated lower blood flows (2.44 ± 0.23 versus 3.43 ± 0.30 mL/min/g; P < 0.05) and reduced wall thickening (33 ± 9% versus 52 ± 13%; P < 0.05) compared with remote regions. RHM had lower respiratory control indices (3.7 ± 0.3 versus 4.3 ± 0.4; P < 0.05) with persistently increased UCP-2 content.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalThe Journal of surgical research
Volume193
Issue number1
DOIs
StatePublished - Jan 1 2015

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health Grant R01HL089307 (E.O.M.), and VA Merit Review Grants (R.F.K.).

Publisher Copyright:
Copyright © 2015 Elsevier Inc. All rights reserved.

Keywords

  • Coronary Artery Bypass Grafting
  • Hibernating Myocardium
  • Mitochondria
  • Revascularization
  • Uncoupling Protein-2

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