Expression of the ras-related rab3a gene in human insulinomas and normal human pancreatic islets

B. Lankat-Buttgereit, H. C. Fehmann, B. J. Hering, R. G. Bretzel, B. Göke

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

To determine the expression of the small-molecular-weight guanosine 5'-triphosphate (GTP)-binding protein rab3a in human endocrine pancreatic tissue, total RNA was isolated from five different human insulinomas and from normal human islets. The expression of rab3a was analyzed by Northern blots utilizing a 1,300-bp Eco RI fragment of the cDN A coding for human rab3a. As in the brain, a specific transcript of 1,800 bp was detected in all insulinomas, though with varying sig-nal intensity. When the northern membranes were rehy-bridized with a radioactively labeled insulin probe, a signal was found for all five insulinomas and cells of normal islets. In contrast, normal human islets showed no detectable expression of rab3a. In conclusion, rab3a is expressed to a significant degree in human insulinoma tissue but not in normal islets.

Original languageEnglish (US)
Pages (from-to)434-438
Number of pages5
JournalPancreas
Volume9
Issue number4
DOIs
StatePublished - Jul 1994

Keywords

  • GTP-binding proteins
  • Human islets
  • Insulinoma
  • Rab3a

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