Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N6-monomethylation and N6,N6-dimethylation of ribosomal RNA

Leonard Katz, David Brown, Kathleen Boris, James Tuan

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6 dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.

Original languageEnglish (US)
Pages (from-to)319-325
Number of pages7
JournalGene
Volume55
Issue number2-3
DOIs
StatePublished - 1987

Keywords

  • Recombinant DNA
  • adenine methylation
  • erythromycin resistance
  • heterologous gene expression
  • in vitro assay
  • phage γ vector

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