The Commelina yellow mottle virus (CoYMV) infects the monocot weed Commelina diffusa. The objective of this study was to investigate the transgene expression conferred by the CoYMV promoter in a monocot species. Friable, embryogenic oat (Avena sativa L.) tissue cultures were stably transformed with the CoYMV promoter fused to the coding region of E. coli β-glucuronidase (uidA, GUS). Developmental and tissue-specific expression of the CoYMV-GUS construct was investigated in regenerated plants and their progeny. Histochemical GUS staining was primarily localized in the vascular tissues of shoots, leaves, floral bracts and in roots. While ovaries stained intensely, no staining was detected in anthers or the endosperm in mature seed. The scutellum of mature and germinating seed exhibited GUS activity.
Bibliographical noteFunding Information:
Supported in part by grants from Quaker Oats Co., Northrup King Co., and The Midwest Plant Biotechnology Consortium, USDA Subgrant 5930130-10. Minnesota Agricultural Experiment Station Publ No. 971130008 Communicated by I. K. Vasil K. A. Torbert · D. A. Somers (½) Department of Agronomy and Plant Genetics, 411 Borlaug Hall 1991 Upper Buford Circle, University of Minnesota, St. Paul, MN55108, USA M. Gopalraj · S. L. Medberry1 · N. E. Olszewski Department of Plant Biology, Plant Molecular Genetics Institute, University of Minnesota, St. Paul, MN 55108, USA
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