TY - JOUR
T1 - Expression of select function associated antigens during pregnancy
AU - Coyne, Bonnie A.
AU - Crombleholme, William R.
AU - Smith, Janis P.
AU - Landers, Daniel V.
PY - 1995
Y1 - 1995
N2 - Pregnancy has long been associated with alterations in phenotypic and functional characteristics of the immune response. We studied a variety of T lymphocyte subsets defined by specific antigen expression during pregnancy using a fluorescence activated cell sorter (FACS) and fluorescein conjugated monoclonal antibodies. Specifically, we performed a cross-sectional analysis of the peripheral blood mononuclear cells from 20 women in the first trimester, 20 women in the second trimester, 26 women in the third trimester and 20 women at the time of delivery. We analyzed for antibody staining of CD3, CD4, CD25 (IL-2 receptor), T cell receptor (beta chain), T cell receptor (delta chain) and two color staining for CD3/CD18 (LFA-1), CD3/CD56 and CD4/CD45R. There was little change in the numbers of cells staining for these antibodies through the three trimesters and at delivery with the exception of T lymphocytes bearing the LFA-1 antigen which were significantly less in the first trimester (P < 0.05). These data support the notion that T cell subsets related to T cell activation, foreign antigen recognition and lymphocyte function are not significantly altered in pregnancy. This supports the observation that immunocompetence is maintained during pregnancy despite the success of the fetal allograft. Definitive conclusions must await longitudinal studies of these antigens.
AB - Pregnancy has long been associated with alterations in phenotypic and functional characteristics of the immune response. We studied a variety of T lymphocyte subsets defined by specific antigen expression during pregnancy using a fluorescence activated cell sorter (FACS) and fluorescein conjugated monoclonal antibodies. Specifically, we performed a cross-sectional analysis of the peripheral blood mononuclear cells from 20 women in the first trimester, 20 women in the second trimester, 26 women in the third trimester and 20 women at the time of delivery. We analyzed for antibody staining of CD3, CD4, CD25 (IL-2 receptor), T cell receptor (beta chain), T cell receptor (delta chain) and two color staining for CD3/CD18 (LFA-1), CD3/CD56 and CD4/CD45R. There was little change in the numbers of cells staining for these antibodies through the three trimesters and at delivery with the exception of T lymphocytes bearing the LFA-1 antigen which were significantly less in the first trimester (P < 0.05). These data support the notion that T cell subsets related to T cell activation, foreign antigen recognition and lymphocyte function are not significantly altered in pregnancy. This supports the observation that immunocompetence is maintained during pregnancy despite the success of the fetal allograft. Definitive conclusions must await longitudinal studies of these antigens.
KW - Monoclonal antibodies
KW - Pregnancy
KW - T lymphocyte antigens
KW - T lymphocyte subsets
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U2 - 10.3109/14767059509017310
DO - 10.3109/14767059509017310
M3 - Article
AN - SCOPUS:0029004387
SN - 1476-7058
VL - 4
SP - 115
EP - 119
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
IS - 3
ER -