Expression of periplasmic chaperones in salmonella typhimurium reduces its viability in vivo

Michael John Mertensotto, Jeremy J. Drees, Lance B. Augustin, Janet L. Schottel, Daniel A. Saltzman

Research output: Contribution to journalArticlepeer-review


The efficacy of live attenuated bacterial vectors is dependent upon the fine-tuning of a strain’s immu-nogenicity and its virulence. Strains are often engineered to deliver heterologous antigens, but soluble expression of recombinant proteins can be troublesome. Therefore, secretion systems or chaperone proteins are routinely used to assist in attaining high levels of functional, soluble protein production. However, the effects of chaperone expression on the virulence of attenuated bacterial vectors have not been previously reported. In anticipation of utilizing periplasmic chaperone proteins to facilitate soluble production of immunomodulatory proteins in an attenuated strain of Salmonella Typhimurium, the production of the chaperones was tested for their effect on both culture growth and bacterial persistence in mouse tissues. Although no effect on growth of the bacteria was observed in vitro, the increased expression of the periplasmic chaperones resulted in over-attenuation of the Salmonella in vivo.

Original languageEnglish (US)
Pages (from-to)433-435
Number of pages3
JournalCurrent Microbiology
Issue number3
StatePublished - Nov 29 2014

Bibliographical note

Funding Information:
The authors are grateful to Arnold Leonard for his experimental advice and critical review of the manuscript. We thank the Minnesota Supercomputing Institute for providing licenses for software used during this research. This study was financially supported by the ASL Cancer Research Fund and

Publisher Copyright:
© Springer Science+Business Media New York 2014.


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