Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Mary S Pampusch; Janet R. Serie; Mark A. Osinski; Virginia S Seybold; Michael P Murtaugh; David R Brown

doi:10.1016/S0196-9781(00)00332-6

Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Mary S Pampusch
, Janet R. Serie
, Mark A. Osinski
, Virginia S Seybold
, Michael P Murtaugh
, David R Brown

Research output: Contribution to journal › Article › peer-review

Abstract

This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [³H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

Original language	English (US)
Pages (from-to)	1865-1870
Number of pages	6
Journal	Peptides
Volume	21
Issue number	12
DOIs	https://doi.org/10.1016/S0196-9781(00)00332-6
State	Published - Dec 1 2000

Keywords

Intestine
Pig
Rat
Spleen
mRNA-protein mismatch

Publisher link

10.1016/S0196-9781(00)00332-6

Find It

Find It at U of M Libraries

Cite this

@article{95b3544402df4ba2ae3f7e0b71183e6a,

title = "Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues",

abstract = "This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.",

keywords = "Intestine, Pig, Rat, Spleen, mRNA-protein mismatch",

author = "Pampusch, \{Mary S\} and Serie, \{Janet R.\} and Osinski, \{Mark A.\} and Seybold, \{Virginia S\} and Murtaugh, \{Michael P\} and Brown, \{David R\}",

year = "2000",

month = dec,

day = "1",

doi = "10.1016/S0196-9781(00)00332-6",

language = "English (US)",

volume = "21",

pages = "1865--1870",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "12",

}

TY - JOUR

T1 - Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

AU - Pampusch, Mary S

AU - Serie, Janet R.

AU - Osinski, Mark A.

AU - Seybold, Virginia S

AU - Murtaugh, Michael P

AU - Brown, David R

PY - 2000/12/1

Y1 - 2000/12/1

N2 - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

AB - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

KW - Intestine

KW - Pig

KW - Rat

KW - Spleen

KW - mRNA-protein mismatch

UR - https://www.scopus.com/pages/publications/0034861122

UR - http://www.scopus.com/inward/citedby.url?scp=0034861122&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(00)00332-6

DO - 10.1016/S0196-9781(00)00332-6

M3 - Article

SN - 0196-9781

VL - 21

SP - 1865

EP - 1870

JO - Peptides

JF - Peptides

IS - 12

ER -

Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this