Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

M. S. Pampusch; J. R. Serie; M. A. Osinski; V. S. Seybold; M. P. Murtaugh; D. R. Brown

doi:10.1016/S0196-9781(00)00332-6

Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

M. S. Pampusch, J. R. Serie, M. A. Osinski, V. S. Seybold, M. P. Murtaugh, D. R. Brown

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [³H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

Original language	English (US)
Pages (from-to)	1865-1870
Number of pages	6
Journal	Peptides
Volume	22
Issue number	12
DOIs	https://doi.org/10.1016/S0196-9781(00)00332-6
State	Published - Dec 2000

Bibliographical note

Funding Information:
This research was supported by NIH grants DA-10200 (DRB) and NS-17702 (VSS). M.S.P. was supported by the Training in Psychoneuroimmunology and Substance Abuse grant T32 DA-07239 and M.A.O. was supported by the Neuroscience Training in Drug Abuse Research grant T32 DA-07234. The authors gratefully acknowledge the excellent technical assistance of Lia G. Abrahams and Dr. David R. Linden.

Keywords

Intestine
MRNA-protein mismatch
Pig
Rat
Spleen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0196-9781(00)00332-6

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title = "Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues",

abstract = "This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.",

keywords = "Intestine, MRNA-protein mismatch, Pig, Rat, Spleen",

author = "Pampusch, {M. S.} and Serie, {J. R.} and Osinski, {M. A.} and Seybold, {V. S.} and Murtaugh, {M. P.} and Brown, {D. R.}",

note = "Funding Information: This research was supported by NIH grants DA-10200 (DRB) and NS-17702 (VSS). M.S.P. was supported by the Training in Psychoneuroimmunology and Substance Abuse grant T32 DA-07239 and M.A.O. was supported by the Neuroscience Training in Drug Abuse Research grant T32 DA-07234. The authors gratefully acknowledge the excellent technical assistance of Lia G. Abrahams and Dr. David R. Linden. ",

year = "2000",

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doi = "10.1016/S0196-9781(00)00332-6",

language = "English (US)",

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AU - Pampusch, M. S.

AU - Serie, J. R.

AU - Osinski, M. A.

AU - Seybold, V. S.

AU - Murtaugh, M. P.

AU - Brown, D. R.

N1 - Funding Information: This research was supported by NIH grants DA-10200 (DRB) and NS-17702 (VSS). M.S.P. was supported by the Training in Psychoneuroimmunology and Substance Abuse grant T32 DA-07239 and M.A.O. was supported by the Neuroscience Training in Drug Abuse Research grant T32 DA-07234. The authors gratefully acknowledge the excellent technical assistance of Lia G. Abrahams and Dr. David R. Linden.

PY - 2000/12

Y1 - 2000/12

N2 - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

AB - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

KW - Intestine

KW - MRNA-protein mismatch

KW - Pig

KW - Rat

KW - Spleen

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Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Abstract

Bibliographical note

Keywords

UN SDGs

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