Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

M. S. Pampusch, J. R. Serie, M. A. Osinski, V. S. Seybold, M. P. Murtaugh, D. R. Brown

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

Original languageEnglish (US)
Pages (from-to)1865-1870
Number of pages6
JournalPeptides
Volume22
Issue number12
DOIs
StatePublished - Dec 2000

Bibliographical note

Funding Information:
This research was supported by NIH grants DA-10200 (DRB) and NS-17702 (VSS). M.S.P. was supported by the Training in Psychoneuroimmunology and Substance Abuse grant T32 DA-07239 and M.A.O. was supported by the Neuroscience Training in Drug Abuse Research grant T32 DA-07234. The authors gratefully acknowledge the excellent technical assistance of Lia G. Abrahams and Dr. David R. Linden.

Keywords

  • Intestine
  • MRNA-protein mismatch
  • Pig
  • Rat
  • Spleen

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