Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Mary S Pampusch; Janet R. Serie; Mark A. Osinski; Virginia S Seybold; Michael P Murtaugh; David R Brown

doi:10.1016/S0196-9781(00)00332-6

Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Mary S Pampusch, Janet R. Serie, Mark A. Osinski, Virginia S Seybold, Michael P Murtaugh, David R Brown

Research output: Contribution to journal › Article › peer-review

Abstract

This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [³H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

Original language	English (US)
Pages (from-to)	1865-1870
Number of pages	6
Journal	Peptides
Volume	21
Issue number	12
DOIs	https://doi.org/10.1016/S0196-9781(00)00332-6
State	Published - Dec 1 2000

Keywords

Intestine
Pig
Rat
Spleen
mRNA-protein mismatch

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10.1016/S0196-9781(00)00332-6

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title = "Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues",

abstract = "This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.",

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author = "Pampusch, {Mary S} and Serie, {Janet R.} and Osinski, {Mark A.} and Seybold, {Virginia S} and Murtaugh, {Michael P} and Brown, {David R}",

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T1 - Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

AU - Pampusch, Mary S

AU - Serie, Janet R.

AU - Osinski, Mark A.

AU - Seybold, Virginia S

AU - Murtaugh, Michael P

AU - Brown, David R

PY - 2000/12/1

Y1 - 2000/12/1

N2 - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

AB - This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

KW - Intestine

KW - Pig

KW - Rat

KW - Spleen

KW - mRNA-protein mismatch

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M3 - Article

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EP - 1870

JO - Peptides

JF - Peptides

IS - 12

ER -

Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Abstract

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