Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues

Mary S Pampusch, Janet R. Serie, Mark A. Osinski, Virginia S Seybold, Michael P Murtaugh, David R Brown

Research output: Contribution to journalArticlepeer-review


This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.

Original languageEnglish (US)
Pages (from-to)1865-1870
Number of pages6
Issue number12
StatePublished - Dec 1 2000


  • Intestine
  • Pig
  • Rat
  • Spleen
  • mRNA-protein mismatch


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