Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of Indigo

Burt D. Ensley, Barry J. Ratzkin, Timothy D. Osslund, Mary J. Simon, Lawrence P. Wackett, David T. Gibson

Research output: Contribution to journalArticlepeer-review

430 Scopus citations

Abstract

A fragment of plasmid NAH7 from Pseudomonas putida PpG7 has been cloned and expressed in Escherichia coli HB101. Growth of the recombinant Escherichia coli in nutrient medium results in the formation of indigo. The production of this dye is increased in the presence of tryptophan or indole. Several bacteria that oxidize aromatic hydrocarbons to cis-dihydrodiols also oxidize indole to indigo. The results suggest that indigo formation is due to the combined activities of tryptophanase and naphthalene dioxygenase.

Original languageEnglish (US)
Pages (from-to)167-169
Number of pages3
JournalScience
Volume222
Issue number4620
DOIs
StatePublished - Jan 1 1983

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