Expression of N-myristoyltransferase inhibitor protein and its relationship to c-Src levels in human colon cancer cell lines

Raju V S Rajala, Scott Dehm, Xuguang Bi, Keith Bonham, Rajendra K. Sharma

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Earlier, we have reported that N-myristoyltransferase (NMT) activity is higher in colonic epithelial neoplasms than in normal appearing colonic tissue and that increase in NMT activity appears at an early stage in colonic carcinogenesis. In this study, we demonstrate increased NMT mRNA in well-differentiated adenocarcinomas. NMT and c-Src mRNA levels were generally elevated in a subset of human colon cancer cell lines. Western blotting analysis employing N-myristoyltransferase inhibitory protein (NIP71) antibody demonstrated low levels of NIP71 in high-expressing c-Src cell lines and high levels of NIP71 in low-expressing c-Src cell lines. Interestingly, down regulation of c-Src by antisense expression in the HT-29 cell line resulted in increased expression of NIP71, suggesting c-Src may negatively regulate NIP71 expression. Furthermore, this is the first study demonstrating the expression of NIP71 in human colon cancer cell lines and a possible relationship to colon carcinogenesis. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1116-1120
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume273
Issue number3
DOIs
StatePublished - Jul 14 2000

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