Earlier, we have reported that N-myristoyltransferase (NMT) activity is higher in colonic epithelial neoplasms than in normal appearing colonic tissue and that increase in NMT activity appears at an early stage in colonic carcinogenesis. In this study, we demonstrate increased NMT mRNA in well-differentiated adenocarcinomas. NMT and c-Src mRNA levels were generally elevated in a subset of human colon cancer cell lines. Western blotting analysis employing N-myristoyltransferase inhibitory protein (NIP71) antibody demonstrated low levels of NIP71 in high-expressing c-Src cell lines and high levels of NIP71 in low-expressing c-Src cell lines. Interestingly, down regulation of c-Src by antisense expression in the HT-29 cell line resulted in increased expression of NIP71, suggesting c-Src may negatively regulate NIP71 expression. Furthermore, this is the first study demonstrating the expression of NIP71 in human colon cancer cell lines and a possible relationship to colon carcinogenesis. (C) 2000 Academic Press.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 14 2000|
Bibliographical noteFunding Information:
This work was partially supported by the Medical Research Council of Canada, and Biomedical Establishment Grant from the Health Services Utilization and Research Commission of Saskatchewan, Canada. We are thankful to Dr. M. P. Walsh, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, for helpful suggestions and critical evaluation of the manuscript.