Expression of kappa opioid receptors in human and monkey lymphocytes

L. F. Chuang, T. K. Chuang, K. F. Killam, Q. Qui, X. R. Wang, J. J. Lin, H. F. Kung, W. Sheng, C. Chao, L. Yu, R. Y. Chuang

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80 Scopus citations


mRNA encoding the kappa opioid receptor gene sequence was identified and isolated from various human lymphocytic cells: CEM x174 (a hybrid of T and B origin) cells, Jurkat-T4 cells, human peripheral blood mononuclear cells (PBMC) and purified CD4+ cells. Analyzing the cDNA sequences of RNA transcripts spanning the putative second extracellular loop, which has reported dynorphin specificity, and the seventh transmembrane domain revealed a 100% and 95% homology in amino acid sequence to corresponding kappa opioid receptor sequences in human placenta and rat brain, respectively. Expression of a similar kappa opioid receptor sequence could be detected in normal monkey PBMC but not in monkey PBMC in which the CD4+/CD8+ cell ratio (or CD4+ cell number) was significantly reduced due to prolonged SIV infection. These findings suggest that human and monkey lymphocytes constitutively express kappa opioid receptor mRNA.

Original languageEnglish (US)
Pages (from-to)1003-1010
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Apr 26 1995


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