Expression of Jun family members in human colorectal adenocarcinoma

Hanlin Wang, Mark P Birkenbach, John Hart

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The products of the Jun family genes, c-Jun, JunB and JunD, are essential components of the activating protein-1 transcription factor complexes that are critically important in the control of cell growth, differentiation and neoplastic transformation. Although increased c-Jun expression has been reported in human colorectal tumors, expression of JunB and JunD in these tumors has not previously been characterized. In the current study, we examined 24 cases of human colorectal adenocarcinoma by western immunoblotting analysis and immunohistochemical staining for the expression of c-Jun, JunB and JunD proteins. Normal-appearing colonic mucosa distant from the tumors in the same colectomy specimens were used as a reference for comparison. The results showed that both c-Jun and JunB proteins were undetectable or barely detectable in normal mucosa but their expression levels were significantly increased in human colorectal adenocarcinomas. In contrast, JunD protein was present at high levels in normal mucosa and only showed a minimal increase in adenocarcinomas. These observations suggest that different Jun proteins may serve different roles in regulating colonic epithelial cell growth and in colorectal tumorigenesis.

Original languageEnglish (US)
Pages (from-to)1313-1317
Number of pages5
JournalCarcinogenesis
Volume21
Issue number7
DOIs
StatePublished - Jan 1 2000

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Adenocarcinoma
jun Genes
Mucous Membrane
Activating Transcription Factor 1
Proteins
Proto-Oncogene Proteins c-jun
Colectomy
Growth
Cell Differentiation
Colorectal Neoplasms
Neoplasms
Carcinogenesis
Western Blotting
Epithelial Cells
Staining and Labeling

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Expression of Jun family members in human colorectal adenocarcinoma. / Wang, Hanlin; Birkenbach, Mark P; Hart, John.

In: Carcinogenesis, Vol. 21, No. 7, 01.01.2000, p. 1313-1317.

Research output: Contribution to journalArticle

Wang, Hanlin ; Birkenbach, Mark P ; Hart, John. / Expression of Jun family members in human colorectal adenocarcinoma. In: Carcinogenesis. 2000 ; Vol. 21, No. 7. pp. 1313-1317.
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