Expression of insulin‐like growth factor binding proteins in human breast cancer correlates with estrogen receptor status

Jose A. Figueroa, James G. Jackson, William L. McGuire, Robert F. Krywicki, Douglas Yee

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The insulin‐like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF‐dependent growth. This study examined the expression of IGFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP‐4 mRNA was detected in all BCCLs studied. IGFBP‐5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP‐6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47‐D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2–6 but none expressed IGFBP‐1 message. IGFBP‐3 expression was higher in ER negative tumors, while that of IGFBP‐4 and ‐5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology.

Original languageEnglish (US)
Pages (from-to)196-205
Number of pages10
JournalJournal of Cellular Biochemistry
Volume52
Issue number2
DOIs
StatePublished - Jan 1 1993

Fingerprint

Insulin-Like Growth Factor Binding Proteins
Estrogen Receptors
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Tumors
Breast Neoplasms
Messenger RNA
Insulin-Like Growth Factor Binding Protein 5
Cells
Insulin-Like Growth Factor Binding Protein 4
Cell Line
Insulin-Like Growth Factor Binding Protein 2
Ligands
Neoplasms
Insulin-Like Growth Factor Binding Protein 6
Ribonucleases
Assays
Growth
Tissue
Proteins

Keywords

  • breast cancer cell lines
  • cell growth
  • hormonal regulation
  • IGFs
  • tumor biology

Cite this

Expression of insulin‐like growth factor binding proteins in human breast cancer correlates with estrogen receptor status. / Figueroa, Jose A.; Jackson, James G.; McGuire, William L.; Krywicki, Robert F.; Yee, Douglas.

In: Journal of Cellular Biochemistry, Vol. 52, No. 2, 01.01.1993, p. 196-205.

Research output: Contribution to journalArticle

Figueroa, Jose A. ; Jackson, James G. ; McGuire, William L. ; Krywicki, Robert F. ; Yee, Douglas. / Expression of insulin‐like growth factor binding proteins in human breast cancer correlates with estrogen receptor status. In: Journal of Cellular Biochemistry. 1993 ; Vol. 52, No. 2. pp. 196-205.
@article{b75b0e67ae174caca54fbb8cb7e299fb,
title = "Expression of insulin‐like growth factor binding proteins in human breast cancer correlates with estrogen receptor status",
abstract = "The insulin‐like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF‐dependent growth. This study examined the expression of IGFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP‐4 mRNA was detected in all BCCLs studied. IGFBP‐5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP‐6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47‐D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2–6 but none expressed IGFBP‐1 message. IGFBP‐3 expression was higher in ER negative tumors, while that of IGFBP‐4 and ‐5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology.",
keywords = "breast cancer cell lines, cell growth, hormonal regulation, IGFs, tumor biology",
author = "Figueroa, {Jose A.} and Jackson, {James G.} and McGuire, {William L.} and Krywicki, {Robert F.} and Douglas Yee",
year = "1993",
month = "1",
day = "1",
doi = "10.1002/jcb.240520211",
language = "English (US)",
volume = "52",
pages = "196--205",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of insulin‐like growth factor binding proteins in human breast cancer correlates with estrogen receptor status

AU - Figueroa, Jose A.

AU - Jackson, James G.

AU - McGuire, William L.

AU - Krywicki, Robert F.

AU - Yee, Douglas

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The insulin‐like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF‐dependent growth. This study examined the expression of IGFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP‐4 mRNA was detected in all BCCLs studied. IGFBP‐5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP‐6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47‐D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2–6 but none expressed IGFBP‐1 message. IGFBP‐3 expression was higher in ER negative tumors, while that of IGFBP‐4 and ‐5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology.

AB - The insulin‐like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF‐dependent growth. This study examined the expression of IGFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP‐4 mRNA was detected in all BCCLs studied. IGFBP‐5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP‐6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47‐D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2–6 but none expressed IGFBP‐1 message. IGFBP‐3 expression was higher in ER negative tumors, while that of IGFBP‐4 and ‐5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology.

KW - breast cancer cell lines

KW - cell growth

KW - hormonal regulation

KW - IGFs

KW - tumor biology

UR - http://www.scopus.com/inward/record.url?scp=0027159591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027159591&partnerID=8YFLogxK

U2 - 10.1002/jcb.240520211

DO - 10.1002/jcb.240520211

M3 - Article

VL - 52

SP - 196

EP - 205

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 2

ER -