Expression of HIV receptors, alternate receptors and co-receptors on tonsillar epithelium

Implications for HIV binding and primary oral infection

Renu B. Kumar, Diane M. Maher, Mark C. Herzberg, Peter J. Southern

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Primary HIV infection can develop from exposure to HIV in the oral cavity. In previous studies, we have documented rapid and extensive binding of HIV virions in seminal plasma to intact mucosal surfaces of the palatine tonsil and also found that virions readily penetrated beneath the tissue surfaces. As one approach to understand the molecular interactions that support HIV virion binding to human mucosal surfaces, we have examined the distribution of the primary HIV receptor CD4, the alternate HIV receptors heparan sulfate proteoglycan (HS) and galactosyl ceramide (GalCer) and the co-receptors CXCR4 and CCR5 in palatine tonsil. Results: Only HS was widely expressed on the surface of stratified squamous epithelium. In contrast, HS, GalCer, CXCR4 and CCR5 were all expressed on the reticulated epithelium lining the tonsillar crypts. We have observed extensive variability, both across tissue sections from any tonsil and between tonsils, in the distribution of epithelial cells expressing either CXCR4 or CCR5 in the basal and suprabasal layers of stratified epithelium. The general expression patterns of CXCR4, CCR5 and HS were similar in palatine tonsil from children and adults (age range 3-20). We have also noted the presence of small clusters of lymphocytes, including CD4+ T cells within stratified epithelium and located precisely at the mucosal surfaces. CD4+ T cells in these locations would be immediately accessible to HIV virions. Conclusion: In total, the likelihood of oral HIV transmission will be determined by macro and micro tissue architecture, cell surface expression patterns of key molecules that may bind HIV and the specific properties of the infectious inoculum.

Original languageEnglish (US)
Article number25
JournalVirology Journal
Volume3
DOIs
StatePublished - Apr 6 2006

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HIV Receptors
Palatine Tonsil
Heparan Sulfate Proteoglycans
Epithelium
HIV
Virion
Infection
Galactosylceramides
CCR5 Receptors
CXCR4 Receptors
T-Lymphocytes
Semen
HIV Infections
Mouth
Epithelial Cells
Lymphocytes

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Expression of HIV receptors, alternate receptors and co-receptors on tonsillar epithelium : Implications for HIV binding and primary oral infection. / Kumar, Renu B.; Maher, Diane M.; Herzberg, Mark C.; Southern, Peter J.

In: Virology Journal, Vol. 3, 25, 06.04.2006.

Research output: Contribution to journalArticle

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