Anucleate minicells produced by a mutated strain of Escherichia coli remain metabolically active for up to 48 h at 37°C. Minicells of increasing age have been infected with the coliphage T7. Infection results in the onset of transcription and translation producing T7 encoded polypeptides. Quantitative and qualitative changes in T7 gene expression result from infection of increasingly old minicells. There is no detectable increase in the frequency of error occurrence in the synthesis of T7 polypeptides in infected old minicells as compared to infected young minicells.
Bibliographical noteFunding Information:
provided very valuable technical assistance. This work was supported by grants IR01AG001546 from the National Institute on Aging and AFOSR-81-0087 from the Air Force Office of Scientific Research. J.N.R. is the recipient of Research ('aree; Development Award 5K04AG00108 from the National Institute for Health on Aging.