Expression of apical Na+–l-glutamine co-transport activity, B0-system neutral amino acid co-transporter (B0AT1) and angiotensin-converting enzyme 2 along the jejunal crypt–villus axis in young pigs fed a liquid formula

Chengbo Yang, Xiaojian Yang, Dale Lackeyram, Todd C. Rideout, Zirong Wang, Barbara Stoll, Yulong Yin, Douglas G. Burrin, Ming Z. Fan

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Gut apical amino acid (AA) transport activity is high at birth and during suckling, thus being essential to maintain luminal nutrient-dependent mucosal growth through providing AA as essential metabolic fuel, substrates and nutrient stimuli for cellular growth. Because system-B0 Na+–neutral AA co-transporter (B0AT1, encoded by the SLC6A19 gene) plays a dominant role for apical uptake of large neutral AA including l-Gln, we hypothesized that high apical Na+–Gln co-transport activity, and B0AT1 (SLC6A19) in co-expression with angiotensin-converting enzyme 2 (ACE2) were expressed along the entire small intestinal crypt–villus axis in young animals via unique control mechanisms. Kinetics of Na+–Gln co-transport activity in the apical membrane vesicles, prepared from epithelial cells sequentially isolated along the jejunal crypt–villus axis from liquid formula-fed young pigs, were measured with the membrane potential being clamped to zero using thiocyanate. Apical maximal Na+–Gln co-transport activity was much higher (p < 0.05) in the upper villus cells than in the middle villus (by 29 %) and the crypt (by 30 %) cells, whereas Na+–Gln co-transport affinity was lower (p < 0.05) in the upper villus cells than in the middle villus and the crypt cells. The B0AT1 (SLC6A19) mRNA abundance was lower (p < 0.05) in the crypt (by 40–47 %) than in the villus cells. There were no significant differences in B0AT1 and ACE2 protein abundances on the apical membrane among the upper villus, the middle villus and the crypt cells. Our study suggests that piglet fast growth is associated with very high intestinal apical Na+–neutral AA uptake activities via abundantly co-expressing B0AT1 and ACE2 proteins in the apical membrane and by transcribing the B0AT1 (SLC6A19) gene in the epithelia along the entire crypt–villus axis.

Original languageEnglish (US)
Pages (from-to)1491-1508
Number of pages18
JournalAmino Acids
Volume48
Issue number6
DOIs
StatePublished - Jun 1 2016

Bibliographical note

Funding Information:
This work was supported by research grants from the Natural Sciences and Engineering Research Council (NSERC) of Canada – Discovery Program and the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) – University of Guelph Partnership’s Animal Research Program (to M.Z. Fan). This work was also partially supported by research grants from USDA/ARS under Cooperative Agreements 58-6250-6-001/the National Institute of Health R01-HD33920 (to D.G. Burrin). Fan M.Z. was supported by a USDA/ARS postdoctoral research fellowship when the project was initiated at the Baylor College of Medicine, Houston, TX. Yang C. was supported by a visiting scholarship from the Chinese Academy of Sciences and Wang Z. was supported by a visiting scholarship from the Chinese Ministry of Education while participating in related research activities in Guelph, ON, Canada. We are grateful to Professor François Verrey from the University of Zurich to provide the rabbit–anti–mouse BAT1 for this research. 0

Publisher Copyright:
© 2016, Springer-Verlag Wien.

Keywords

  • Angiotensin-converting enzyme 2
  • Gut crypt–villus axis
  • SLC6A19
  • Small intestinal apical l-glutamine uptake
  • System-B Na–neutral amino acid co-transporter

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