Expression of a heat-inducible gene in transfected mosquito cells

Anna Gerenday, Park Yang-Ja Park, Lan Que Lan, Ann M Fallon

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Abstract

The evolutionary conservation of the heat shock response suggests that plasmids containing promoters from Drosophila heat shock protein (hsp) genes will be useful in the development of gene transfer procedures for cell lines representing a variety of insect species. Conditions for induction of endogenous hsp genes and for expression of the chloramphenicol acetyltransferase (CAT) gene regulated by the Drosophila hsp 70 promoter were examined in Aedes albopictus (mosquito) cells. Five hsps, ranging in size from 27,000 to 90,000 D, were induced in A. albopictus cells during incubation at 41°C in medium containing [35S]methionine. Relative synthesis of these proteins at 37 and 41°C indicated that Aedes hsp 66 is homologous to Drosophila hsp 70. Detection of CAT activity in transfected mosquito cells was enhanced 10-fold under heat shock conditions (6 h, 41°C) based on maximal expression of hsp 66, relative to conditions defined for expression of hsp 70 in Drosophila cells. Analysis of the endogenous heat shock response may be essential to the optimal use of plasmids containing the Drosophila hsp 70 promoter with other insect cell types.

Original languageEnglish (US)
Pages (from-to)679-686
Number of pages8
JournalInsect Biochemistry
Volume19
Issue number7
DOIs
StatePublished - 1989

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Keywords

  • Aedes albopictus
  • cultured mosquito cells
  • gene transfer
  • heat shock response

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