Expression of α6 and β4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin

Amy P Skubitz, Robert C. Bast, Elizabeth A. Wayner, Paul C. Letourneau, Mark S. Wilke

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins α1β1, α2β1, α3β1, α6β1, and α6β4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for β1, α2, and α3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for α6 and β4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for β1, α2, and α3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for α6 and β4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for α6 and β4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the α6 and β4 integrin subunits. As α6 and β4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of α6 and β4 integrin subunits.

Original languageEnglish (US)
Pages (from-to)1445-1461
Number of pages17
JournalAmerican Journal of Pathology
Volume148
Issue number5
StatePublished - May 1 1996

Fingerprint

Laminin
Basement Membrane
Integrins
Membrane Proteins
Epithelial Cells
Carcinoma
Ovary
Ascites
Neoplasms
Laminin Receptors
Staining and Labeling
Phenotype
Cell Surface Receptors
Glycoproteins
Proteins

Cite this

Expression of α6 and β4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. / Skubitz, Amy P; Bast, Robert C.; Wayner, Elizabeth A.; Letourneau, Paul C.; Wilke, Mark S.

In: American Journal of Pathology, Vol. 148, No. 5, 01.05.1996, p. 1445-1461.

Research output: Contribution to journalArticle

Skubitz, Amy P ; Bast, Robert C. ; Wayner, Elizabeth A. ; Letourneau, Paul C. ; Wilke, Mark S. / Expression of α6 and β4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. In: American Journal of Pathology. 1996 ; Vol. 148, No. 5. pp. 1445-1461.
@article{150d2a48c48b4344b1229dbdcb09478c,
title = "Expression of α6 and β4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin",
abstract = "The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins α1β1, α2β1, α3β1, α6β1, and α6β4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for β1, α2, and α3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for α6 and β4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for β1, α2, and α3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for α6 and β4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for α6 and β4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the α6 and β4 integrin subunits. As α6 and β4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of α6 and β4 integrin subunits.",
author = "Skubitz, {Amy P} and Bast, {Robert C.} and Wayner, {Elizabeth A.} and Letourneau, {Paul C.} and Wilke, {Mark S.}",
year = "1996",
month = "5",
day = "1",
language = "English (US)",
volume = "148",
pages = "1445--1461",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Expression of α6 and β4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin

AU - Skubitz, Amy P

AU - Bast, Robert C.

AU - Wayner, Elizabeth A.

AU - Letourneau, Paul C.

AU - Wilke, Mark S.

PY - 1996/5/1

Y1 - 1996/5/1

N2 - The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins α1β1, α2β1, α3β1, α6β1, and α6β4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for β1, α2, and α3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for α6 and β4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for β1, α2, and α3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for α6 and β4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for α6 and β4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the α6 and β4 integrin subunits. As α6 and β4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of α6 and β4 integrin subunits.

AB - The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins α1β1, α2β1, α3β1, α6β1, and α6β4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for β1, α2, and α3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for α6 and β4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for β1, α2, and α3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for α6 and β4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for α6 and β4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the α6 and β4 integrin subunits. As α6 and β4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of α6 and β4 integrin subunits.

UR - http://www.scopus.com/inward/record.url?scp=0029915884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029915884&partnerID=8YFLogxK

M3 - Article

C2 - 8623916

AN - SCOPUS:0029915884

VL - 148

SP - 1445

EP - 1461

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 5

ER -